Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases.

Abstract

The NEK1 kinase controls ciliogenesis, mitosis, and DNA repair, and NEK1 mutations cause human diseases including axial spondylometaphyseal dysplasia and amyotrophic lateral sclerosis. C21ORF2 mutations cause a similar pattern of human diseases, suggesting close functional links with NEK1 Here, we report that endogenous NEK1 and C21ORF2 form a tight complex in human cells. A C21ORF2 interaction domain "CID" at the C-terminus of NEK1 is necessary for its association with C21ORF2 in cells, and pathogenic mutations in this region disrupt the complex. AlphaFold modelling predicts an extended binding interface between a leucine-rich repeat domain in C21ORF2 and the NEK1-CID, and our model may explain why pathogenic mutations perturb the complex. We show that NEK1 mutations that inhibit kinase activity or weaken its association with C21ORF2 severely compromise ciliogenesis, and that C21ORF2, like NEK1 is required for homologous recombination. These data enhance our understanding of how the NEK1 kinase is regulated, and they shed light on NEK1-C21ORF2-associated diseases.

• Authors: Mateusz Gregorczyk, Graziana Pastore, Ivan Muñoz, Thomas Carroll, Johanna Streubel, Meagan Munro, Pawel Lis, Sven Lange, Frederic Lamoliatte, Thomas Macartney, Rachel Toth, Fiona Brown, James Hastie, Gislene Pereira, Daniel Durocher, John Rouse
• Journal: Life science alliance (Life Sci Alliance) Vol. 6 Issue 7 (07 2023) ISSN: 2575-1077 [Electronic] United States
• PMID: 37188479 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2023 Gregorczyk et al.

Chemical References

• NEK1 protein, human
• NIMA-Related Kinase 1
• CFAP410 protein, human

Topics

• Humans
• DNA Repair
• Mutation (genetics)
• NIMA-Related Kinase 1 (genetics)
• Osteochondrodysplasias (genetics)
• Phosphorylation