Prevention of dextran sulfate sodium (DSS)-induced ulcerative colitis in mice by a synbiotic approach using probiotic and lactulose.

Abstract

In this research, the synbiotic effects of the probiotic Lactiplantibacillus plantarum YW11 and lactulose on intestinal morphology, colon function, and immune activity were evaluated in a mouse model of UC induced by dextran sulfate sodium (DSS). The results revealed that L. plantarum YW11 in combination with lactulose decreased the severity of colitis in mice and improved the structure of the damaged colon, as assessed using colon length and disease condition. Moreover, colonic levels of pro-inflammatory cytokines (IL-1β, IL-6, IL-12, TNF-α, and IFN-γ) were significantly lower and anti-inflammatory factors (IL-10) were significantly higher following the synbiotic supplementation. The synbiotic also exerted antioxidant effects by up-regulating SOD and CAT levels and down-regulating MDA levels in colon tissue. It could also reduce the relative expression of iNOS mRNA and increase the relative expression of nNOS and eNOS mRNA. Western blot confirmed the increased expression of c-Kit, IκBα, and SCF and significantly reduced expression of the NF-κB protein. Therefore, the combination of L. plantarum YW11 and lactulose exerted therapeutic effects mainly through the NF-κB anti-inflammatory pathway, which represented a novel synbiotic approach in the prevention of colonic inflammation.

• Authors: Gege Hu, Shaoli Liu, Hong Zeng, Ruokun Yi, Xin Zhao, Zhennai Yang
• Journal: Letters in applied microbiology (Lett Appl Microbiol) Vol. 76 Issue 5 (May 02 2023) ISSN: 1472-765X [Electronic] England
• PMID: 37133416 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.

Chemical References

• Lactulose
• NF-kappa B
• Dextran Sulfate
• Anti-Inflammatory Agents

Topics

• Animals
• Mice
• Colitis, Ulcerative (chemically induced, drug therapy, prevention & control)
• Lactulose (metabolism, pharmacology, therapeutic use)
• NF-kappa B (genetics, metabolism)
• Dextran Sulfate (toxicity, metabolism)
• Synbiotics
• Colon (metabolism)
• Anti-Inflammatory Agents (therapeutic use)
• Probiotics (therapeutic use)
• Disease Models, Animal
• Mice, Inbred C57BL