In Vitro Cell Proliferation and Migration Properties of Oral Mucosal Fibroblasts: A Comparative Study on the Effects of Cord Blood- and Peripheral Blood-Platelet Lysate.
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| Abstract |
Cord blood-platelet lysate (CB-PL), containing growth factors such as a platelet-derived growth factor, has a similar efficacy to peripheral blood-platelet lysate (PB-PL) in initiating cell growth and differentiation, which makes it a unique alternative to be implemented into oral ulceration healing. This research study aimed to compare the effectiveness of CB-PL and PB-PL in promoting oral wound closure in vitro. Alamar blue assay was used to determine the optimal concentration of CB-PL and PB-PL in enhancing the proliferation of human oral mucosal fibroblasts (HOMF). The percentage of wound closure was measured using the wound-healing assay for CB-PL and PB-PL at the optimal concentration of 1.25% and 0.3125%, respectively. The gene expressions of cell phenotypic makers (Col. I, Col. III, elastin and fibronectin) were determined via qRT-PCR. The concentrations of PDGF-BB were quantified using ELISA. We found that CB-PL was as effective as PB-PL in promoting wound-healing and both PL were more effective compared to the control (CTRL) group in accelerating the cell migration in the wound-healing assay. The gene expressions of Col. III and fibronectin were significantly higher in PB-PL compared to CB-PL. The PDGF-BB concentration of PB-PL was the highest and it decreased after the wound closed on day 3. Therefore, we concluded that PL from both sources can be a beneficial treatment for wound-healing, but PB-PL showed the most promising wound-healing properties in this study.
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| Authors | Arief Faisal Azmi, Mohammad Amirul Asyraff Mohd Yahya, Nur Ain Azhar, Norliwati Ibrahim, Norzana Abd Ghafar, Nur Azurah Abdul Ghani, Muhammad Aiman Mohd Nizar, Siti Salmiah Mohd Yunus, Tashveender Kaur Lakhbir Singh, Jia-Xian Law, Sook-Luan Ng |
| Journal | International journal of molecular sciences (Int J Mol Sci) Vol. 24 Issue 6 (Mar 17 2023) ISSN: 1422-0067 [Electronic] Switzerland |
| PMID | 36982842 (Publication Type: Journal Article) |
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