Abstract |
Given the widespread use of point-of-care testing for diagnosis of disease, micro-scale electrochemical deoxyribonucleic acid ( DNA) biosensors have become a promising area of research owing to its fast mass transfer, high current density and rapid response. In this study, a gold nanoparticles modified gold microelectrode (AuNPs/Au-Me) was constructed to determine the hand, foot and mouth disease (HFMD)-related gene. The noble metal nanoparticles modification yielded ca. 7.4-fold increase in electroactive surface area of microelectrode, and the signal for HFMD-related gene was largely magnified. Under optimal conditions, the biosensor exhibited salient selectivity and sensitivity with a low detection limit of 0.3 fM (S/N = 3), which is sufficient for clinical diagnosis of HFMD. Additionally, the developed AuNPs/Au-Me was successfully applied to determining the polymerase chain reaction (PCR) amplified products of target gene. Thus, the electrochemical DNA biosensor possesses great potential in early-stage diagnosis and long-term monitoring of various disease.
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Authors | Yu Zhong, Xiang-Guang Hu, Ai-Lin Liu, Yun Lei |
Journal | Analytica chimica acta
(Anal Chim Acta)
Vol. 1252
Pg. 341034
(Apr 29 2023)
ISSN: 1873-4324 [Electronic] Netherlands |
PMID | 36935134
(Publication Type: Journal Article)
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Copyright | Copyright © 2023 Elsevier B.V. All rights reserved. |
Chemical References |
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Topics |
- Humans
- Gold
- Microelectrodes
- Hand, Foot and Mouth Disease
(diagnosis)
- Metal Nanoparticles
- DNA
(genetics)
- Biosensing Techniques
- Electrochemical Techniques
- Limit of Detection
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