Evaluation of the safety, immunogenicity and efficacy of a new live-attenuated lumpy skin disease vaccine in India.

Abstract

Lumpy skin disease (LSD) was reported for the first time in India in 2019 and since then, it has become endemic. Since a homologous (LSD-virus based) vaccine was not available in the country, goatpox virus (GPV)-based heterologous vaccine was authorized for mass immunization to induce protection against LSD in cattle. This study describes the evaluation of safety, immunogenicity and efficacy of a new live-attenuated LSD vaccine developed by using an Indian field strain, isolated in 2019 from cattle. The virus was attenuated by continuous passage (P = 50) in Vero cells. The vaccine (50th LSDV passage in Vero cells, named as Lumpi-ProVacInd) did not induce any local or systemic reaction upon its experimental inoculation in calves (n = 10). At day 30 post-vaccination (pv), the vaccinated animals were shown to develop antibody- and cell-mediated immune responses and exhibited complete protection upon virulent LSDV challenge. A minimum Neethling response (0.018% animals; 5 out of 26,940 animals) of the vaccine was observed in the field trials conducted in 26,940 animals. There was no significant reduction in the milk yield in lactating animals (n = 10108), besides there was no abortion or any other reproductive disorder in the pregnant animals (n = 2889). Sero-conversion was observed in 85.18% animals in the field by day 30 pv.

• Authors: Naveen Kumar, Sanjay Barua, Ram Kumar, Nitin Khandelwal, Amit Kumar, Assim Verma, Lokender Singh, Bhagraj Godara, Yogesh Chander, Garvit Kumar, Thachamvally Riyesh, Deepak Kumar Sharma, Anubha Pathak, Sanjay Kumar, Ramesh Kumar Dedar, Vishal Mehta, Mitesh Gaur, Bhupendra Bhardwaj, Vithilesh Vyas, Sarjeet Chaudhary, Vijaypal Yadav, Adrish Bhati, Rakesh Kaul, Arif Bashir, Anjum Andrabi, Raja Wasim Yousuf, Abhimanyu Koul, Subhash Kachhawaha, Amol Gurav, Siddharth Gautam, Hari Audh Tiwari, Vijay Kumar Munjal, Madhurendu K Gupta, Rajender Kumar, Baldev R Gulati, Jyoti Misri, Ashok Kumar, Ashok Kumar Mohanty, Sukdeb Nandi, Karam Pal Singh, Yash Pal, Triveni Dutt, Bhupendra N Tripathi
• Journal: Virulence (Virulence) Vol. 14 Issue 1 Pg. 2190647 (12 2023) ISSN: 2150-5608 [Electronic] United States
• PMID: 36919498 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Vaccines, Attenuated
• Viral Vaccines

Topics

• Animals
• Cattle
• Female
• Chlorocebus aethiops
• Lumpy Skin Disease (prevention & control, epidemiology)
• Lumpy skin disease virus (genetics)
• Vaccines, Attenuated (adverse effects)
• Vero Cells
• Viral Vaccines (administration & dosage)