Electrostatic assemblies of molecularly imprinted polymers on the surface of electrospun nanofiber membranes for the point-of-care detection of thiodiglycol, a sulfur mustard poisoning metabolic marker.
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| Abstract |
In this study, molecularly imprinted polymers (MIPs) were assembled on the surface of ethylene imine polymer (PEI)/poly(vinyl alcohol) (PVA) electrospun nanofiber membranes for the point-of-care testing (POCT) of thiodiglycol (TDG), a sulfur mustard poisoning metabolic marker, using concentrated gold nanoparticles (AuNPs) as the signal reporting units. The MIPs/PEI/PVA nanofiber membranes could capture TDG specifically through the recognition interaction between MIPs and TDG. Then, AuNPs were adsorbed onto the MIPs/PEI/PVA nanofiber membranes through the Au-S interaction between TDG and AuNPs to produce a visible red color. In order to improve the sensitivity, the silver-enhanced solutions were used to deepen the color of the nanofiber membranes and the software Image J was used to read the gray value as the signal response for subsequent analysis. There was a good linear relationship between the color change of the MIPs/PEI/PVA nanofiber membranes and the TDG concentration from 0.1 ng mL-1 to 1.0 μg mL-1, and the limit of detection was 38 pg mL-1. This method was applied for the selective detection of TDG in urine, showing great potential for the clinical diagnosis of mustard gas poisoning.
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| Authors | Yu Jie Luo, Qi Chao Ye, Tian Jin Xie, Li Li Tian, Yuan Yan, Zhan Lei, Dong Mei Wang, Cheng Zhi Huang, Yuan Fang Li, Shu Jun Zhen |
| Journal | Analytical methods : advancing methods and applications (Anal Methods) Vol. 15 Issue 12 Pg. 1500-1505 (03 23 2023) ISSN: 1759-9679 [Electronic] England |
| PMID | 36916642 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Chemical References |
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