Effect of protection of enoxaparin against experimental ischemia/reperfusion injury in the rat ovary on in vitro fertilization outcomes.

Abstract

OBJECTIVE:

The study aimed to investigate the protection of enoxaparin (E) against experimental ischemic (I) and ischemic-reperfusion (I/R) injury in rat ovaries on in vitro fertilization outcomes.

METHODS:

In total, 56 adult female Sprague-Dawley albino rats were randomly assigned to 6 groups of 8 animals each: Sham, Ischemia, I/R, Sham+E, I+E, and I/R+E. Ischemia groups were subjected to bilateral adnexal torsion for 3 h. In contrast, I/R and I/R+E groups received subsequent detorsion for 3 h. Enoxaparin (0.5 mg/kg s.c.) was administered 30 min prior to ischemia (I+platelet-rich plasma) or reperfusion (I/R+I+platelet-rich plasma). Ovaries were stimulated through intraperitoneal injection of 150-300 internal units IU/kg pregnant mare serum gonadotropin. Anti-Müllerian hormone levels were measured before and after surgery in all groups.

RESULTS:

When the number of metaphase II oocytes was evaluated, statistically significant differences were observed between the I and I+E (p=0.001) and I/R and I/R+E (p=0.000) groups. When both I and I+E groups and I/R and I/R+E groups were compared, it was found that E application increased the number of fertilized oocytes. The number of embryos on the second day was higher in the I/R+E group than that in the I/R group. Statistically significant differences were found in the number of grade 1 embryos between the I/R and I/R+E groups (p=0.003). In comparing anti-Müllerian hormone values within the group, the highest decrease was observed in the I and I/R groups.

CONCLUSION:

Enoxaparin effectively minimizes ovarian damage and preserves ovarian reserve following ovarian torsion.

• Authors: Mehmet Suhha Bostancı, Ozcan Budak, Hüseyin Çakiroğlu, Osman Köse, Özkan Durmaz, Erdem Cokluk
• Journal: Revista da Associacao Medica Brasileira (1992) (Rev Assoc Med Bras (1992)) Vol. 69 Issue 3 Pg. 380-385 ( 2023) ISSN: 1806-9282 [Electronic] Brazil
• PMID: 36820765 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Enoxaparin
• Anti-Mullerian Hormone

Topics

• Animals
• Humans
• Rats
• Female
• Antioxidants
• Ovarian Diseases (drug therapy, prevention & control)
• Enoxaparin (pharmacology, therapeutic use)
• Anti-Mullerian Hormone
• Rats, Sprague-Dawley
• Reperfusion Injury (prevention & control)
• Ischemia
• Fertilization in Vitro