Diagnostic Yield of In-Vitro Vitreous Biopsy for Intraocular Lymphoma at Variable Vitreous Cutter Speeds Using 25-Gauge Vitrectomy.

Abstract

PURPOSE:

Primary vitreoretinal lymphoma (PVRL) is the most common intraocular lymphoproliferative disorder. We evaluated the diagnostic yield of PPV, specifically using modern high cut rate dual-cycle cutters, on in vitro cell viability and diagnostic yield.

METHODS:

Human Burkitt lymphoma cell line Namalwa at 2x10^5 cells/mL was aspirated by 25 gauge (25g) dual-blade guillotine-type vitrectomy at five speeds (500, 1000, 4000, 7500, or 15000 cuts per minute). Cell viability and diagnostic yield in each subtype group were determined using hemocytometry, viable cell count using Cell Counting Kit-8 (CCK-8), and pathologist-guided manual count.

RESULTS:

No significant deviation in cell count was identified in any cut rate by ANOVA (P=0.61), and no trends in the number of viable cells were identified across cut rates (R2 = 0.188, P=0.47). Among histologic cell counts per cut-rate, neither linear regression (R = 0.531, P = 0.16) nor ANOVA (P = 0.096) were statistically significant.

CONCLUSION:

There was no significant degradation in the number of viable cells with increasing cut speed. These results suggest that in contrast to prior findings using 20g or 23g vitrectomy for diagnostic vitrectomy, modern vitrectomy systems may be used at up to 15000 cpm without compromising the viability of lymphoma cells.

• Authors: Tekumalla Sruti, Xu David, Awh Katherine, Philp Nancy, Milman Tatyana, Garg Sunir
• Journal: Retina (Philadelphia, Pa.) (Retina) (Jan 31 2023) ISSN: 1539-2864 [Electronic] United States
• PMID: 36735414 (Publication Type: Journal Article)