Fuc-S-A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide-Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host-Microbe Tryptophan Metabolism.

Abstract

SCOPE:

The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease.

METHODS AND RESULTS:

A polysaccharide named Fuc-S, with a molecular weight of 156 kDa, was prepared by the ultrasonic degradation of fucoidan. Monosaccharide composition, FTIR, methylation, and NMR spectral analysis indicated that Fuc-S may have a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→. Moreover, male C57BL/6 mice were fed three cycles of 1.8% dextran sulfate sodium (DSS) for 5 days and then water for 7 days to induce colitis. The longitudinal microbiome alterations were evaluated using 16S amplicon sequencing. In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice. Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host-microbe tryptophan metabolism of the mice with chronic colitis.

CONCLUSION:

Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.

• Authors: Haitao Xiao, Jinxiu Feng, Jiao Peng, Peigen Wu, Yaoyao Chang, Xianqian Li, Jinhui Wu, Haifeng Huang, Huan Deng, Miao Qiu, Yuedong Yang, Bin Du
• Journal: Marine drugs (Mar Drugs) Vol. 21 Issue 1 (Dec 25 2022) ISSN: 1660-3397 [Electronic] Switzerland
• PMID: 36662189 (Publication Type: Journal Article)

Chemical References

• Dextran Sulfate
• Sulfates
• Tryptophan
• Oligosaccharides

Topics

• Animals
• Male
• Mice
• Colitis (chemically induced, drug therapy, metabolism)
• Colon (metabolism)
• Dextran Sulfate
• Disease Models, Animal
• Gastrointestinal Microbiome
• Mice, Inbred C57BL
• Sulfates (pharmacology)
• Tryptophan (pharmacology)
• Ultrasonics
• Oligosaccharides