Abstract | OBJECTIVE: Fetal surgery for spina bifida aperta (SBA) by open hysterotomy typically repairs anatomical native tissue in layers. Increasingly, fetoscopic repair is performed using a dural patch followed by skin closure. We studied the host response to selected commercially available patches currently being used in a fetal rabbit model for spina bifida repair. METHODS: SBA was surgically induced at 23-24 days of gestation (term = 31 days). Fetal rabbits were assigned to unrepaired (SBA group), or immediate repair with Duragen™ or Durepair™. Non-operated littermates served as normal controls. At term, spinal cords underwent immunohistochemical staining including Nissl and glial fibrillary acidic protein. We hypothesized that spinal cord coverage with a dural patch and skin closure would preserve motor neuron density within the non-inferiority limit of 201.65 cells/mm2 and reduce inflammation compared to unrepaired SBA fetuses. RESULTS: Motor neuron density assessed by Nissl staining was conserved both by Duragen (n = 6, 89.5; 95% CI -158.3 to -20.6) and Durepair (n = 6, 37.0; 95% CI -132.6 to -58.5), whereas density of GFAP-positive cells to quantify inflammation was lower than in unrepaired SBA-fetuses (SBA 2366.0 ± 669.7 cells/mm2 vs. Duragen 1274.0 ± 157.2 cells/mm2 ; p = 0.0002, Durepair 1069.0 ± 270.7 cells/mm2 ; p < 0.0001). CONCLUSIONS: Covering the rabbit spinal cord with either Duragen or Durepair followed by skin closure preserves motor neuron density and reduces the inflammatory response.
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Authors | Yada Kunpalin, Simen Vergote, Luc Joyeux, Onur Telli, Anna L David, Michael Belfort, Paolo De Coppi, Jan Deprest |
Journal | Prenatal diagnosis
(Prenat Diagn)
Vol. 43
Issue 3
Pg. 370-381
(03 2023)
ISSN: 1097-0223 [Electronic] England |
PMID | 36650109
(Publication Type: Journal Article)
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Copyright | © 2023 John Wiley & Sons Ltd. |
Topics |
- Pregnancy
- Female
- Humans
- Animals
- Rabbits
- Spina Bifida Cystica
(surgery)
- Fetus
(surgery)
- Prenatal Care
- Fetoscopy
- Spinal Cord
(surgery)
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