[Myrislignan Induces Apoptosis in Gastric Cancer Cell Line Through PI3K/AKT Signaling Pathway].

Abstract

Objective:

To investigate the effect of myrislignan (MYR) on the apoptosis of gastric cancer cell line and its relationship with phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway.

Methods:

The gastric cells (SGC-7901) were treated with MYR at different concentrations, i.e., 0, 25, 50, 100, and 200 μmol/L, for 48 h and 72 h and the effect of MYR on the proliferation of SGC-7901 cells was measured by CCK-8 assay. Then, SGC-7901 cells were treated with different concentrations of MYR at 50, 100, and 200 μmol/L for 48 h. Meanwhile, a normal control group and a dimethyl sulfoxide (DMSO) solvent control group (0.1% DMSO) were established. Flow cytometry was used to determine the apoptosis rate of SGC-7901 cells. The protein expression levels of PI3K, AKT, Bcl-2-associated X protein (BAX), cysteine-dependent aspartate-specifc protease-3 (Caspase-3), and Caspase-9 were determined by Western blot. Then, PI3K activator (20 μmol/mL) was used to treat SGC-7901 cells for 48 h in 4 groups, the control group, 0.1% DMSO group, MYR group, and MYR+PI3K activator group, and the effect on MYR's induction of apoptosis and regulation of the protein expression levels of PI3K, AKT, BAX, Caspase-3, and Caspase-9 in SGC-7901 cells.

Results:

Compared with the control group, MYR at 50, 100 and 200 μmol/L inhibited the proliferation of gastric cancer cells, increased the apoptosis rate, down-regulated the protein expression levels of PI3K and AKT, and up-regulated the protein expression levels of BAX, Caspase-3, and Caspase-9 in a dose-dependent manner ( P<0.05). However, PI3K activator attenuated MYR-induced apoptosis in gastric cancer cells and MYR's regulation of PI3K, AKT, BAX, Caspase-3, and Caspase-9 protein expression ( P<0.05).

Conclusion:

MYR induces the expression of BAX, Caspase-3, and Caspase-9 proteins by inhibiting the PI3K/AKT signaling pathway, thereby promoting the apoptosis of gastric cancer cells.

• Authors: Yong-Jun Zhou, Yong-Yue Pan, Li-Jun Yang, Ming-Jiang Bie
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 54 Issue 1 Pg. 136-141 (Jan 2023) ISSN: 1672-173X [Print] China
• PMID: 36647656 (Publication Type: English Abstract, Journal Article)
• Copyright: Copyright© by Editorial Board of Journal of Sichuan University (Medical Sciences).

Chemical References

• Proto-Oncogene Proteins c-akt
• Phosphatidylinositol 3-Kinase
• bcl-2-Associated X Protein
• Phosphatidylinositol 3-Kinases
• Caspase 3
• Caspase 9
• 2-(4-allyl-2,6-dimethoxyphenoxy)-1-(4-hydroxy-3-methoxyphenyl)propan-1-ol
• Dimethyl Sulfoxide

Topics

• Humans
• Proto-Oncogene Proteins c-akt (metabolism)
• Phosphatidylinositol 3-Kinase (metabolism)
• bcl-2-Associated X Protein (metabolism)
• Phosphatidylinositol 3-Kinases (metabolism)
• Stomach Neoplasms (metabolism)
• Caspase 3 (metabolism)
• Caspase 9 (metabolism)
• Dimethyl Sulfoxide (pharmacology)
• Cell Proliferation
• Cell Line, Tumor
• Signal Transduction
• Apoptosis