Effects of Ozone Therapy on Chronic Arsenic Poisoning in Rats.

Abstract

Arsenic (As) is a toxic metalloid that affects many organs through drinking water. This study aims to examine the efficacy of ozone therapy on chronic arsenic toxicity. Twenty-four male Wistar albino rats were housed in individual cages and grouped as control, As, O3, and As + O3. As was applied by adding 5 mg/kg/day in drinking water for 60 days. Ozone therapy was applied at 0.5 mg/kg/day (i.p.) O3 in the last 5 days of the experimental period. Tissues were harvested and analyzed for histopathological injury and apoptotic markers. There was no significant difference between the As + O3 and O3 groups (p = 0.186 and p = 0.599) for light microscopic criteria: inflammatory cell infiltration and hydropic degeneration in liver tissue.In TUNEL assessments, similar outcomes were obtained in the control and As + O3 groups. A statistically significant increase was observed in p53 and Caspase 3 (Casp-3) expression levels in the As group compared to the O3 and As + O3 groups. There was no significant difference between the As + O3 and O3 groups on peritubular hemorrhage and desquamation parameters in kidneys (p = 0.147 and p = 0.094). The KIM-1 expression level was significantly increased in the As group compared to the As + O3 group (p = 0.01), and the Casp-3 expression level was not significantly changed in the O3 group compared to the As + O3 group (p = 0.59). In conclusion, it is determined that ozone therapy has ameliorative effects on the microscopic injury of liver and kidney tissues. In addition to microscopic improvement, KIM-1 gene expression levels were ameliorated in the kidneys. The apoptotic cell counts and the Casp-3 and p53 gene expression levels were decreased by O3 administration. Thus, ozone therapy can be a treatment choice for As toxicity.

• Authors: Başak Büyük, Cemre Aydeğer, Özlem Öztopuz, Mehmet Akif Ovalı, Mustafa Makav, Hüseyin Avni Eroğlu
• Journal: Biological trace element research (Biol Trace Elem Res) Vol. 201 Issue 8 Pg. 3951-3960 (Aug 2023) ISSN: 1559-0720 [Electronic] United States
• PMID: 36437433 (Publication Type: Journal Article)
• Copyright: © 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Chemical References

• Ozone
• Arsenic
• Drinking Water
• Tumor Suppressor Protein p53

Topics

• Rats
• Male
• Animals
• Rats, Wistar
• Ozone (pharmacology)
• Arsenic (toxicity)
• Arsenic Poisoning
• Drinking Water
• Tumor Suppressor Protein p53