Abstract | BACKGROUND: Intrawound vancomycin powder is effective in preventing surgical site infection after spine surgery. In a previous study, vancomycin-induced cytotoxicity in osteoblasts was investigated in vitro, and vitamin D3 was verified to be a candidate drug aiding recovery from vancomycin-induced cytotoxicity. The treatment practices involving osteogenesis-promoting drugs vary widely. Teriparatide, an anabolic agent, highly promotes bone formation by inducing osteoblast activation, increasing bone formation and mineral density, and preventing vertebral fractures. Hence, teriparatide may be administered in combination with vancomycin. METHODS: MC3T3-E1 cells were cultured in minimum essential medium supplemented with 10% fetal bovine serum at 37 °C in a humidified incubator containing 5% CO2. The experimental concentrations of vancomycin (2500, 5000, and 7500 μg/mL) were determined based on previous reports and our preliminary experiments. Teriparatide (100 ng/mL) was administered concomitantly to prevent cytotoxicity in osteoblasts, using pulsed vancomycin for 24 h (measured at 1, 3, and 7 days). Cell numbers and morphological changes in cells treated with vancomycin or vancomycin plus 100 ng/mL teriparatide were measured. Osteoblast differentiation was assessed using alkaline phosphatase staining, alkaline phosphatase activity, and alizarin red S staining. RESULTS: CONCLUSION:
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Authors | Kentaro Tsuji, Soichiro Kimura, Kazuhiro Tateda, Hiroshi Takahashi |
Journal | Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association
(J Orthop Sci)
Vol. 28
Issue 6
Pg. 1384-1391
(Nov 2023)
ISSN: 1436-2023 [Electronic] Japan |
PMID | 36371341
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Vancomycin
- Teriparatide
- alizarin
- Alkaline Phosphatase
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Topics |
- Humans
- Vancomycin
(toxicity)
- Teriparatide
(pharmacology, therapeutic use)
- Alkaline Phosphatase
- Cell Differentiation
- Osteogenesis
- Osteoblasts
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