Abstract |
This study aimed to identify novel pancreatic lipase (PL) inhibitors using affinity ultrafiltration combined with spectroscopy and molecular docking. Cyanidin-3-O-glucoside (C3G; IC50: 0.268 mg/mL) and catechin (IC50: 0.280 mg/mL) were shown to be potent PL inhibitors extracted from black rice and adzuki bean coat extracts. Isobologram analysis revealed that the combined use of C3G and catechin at a ratio of 2:3 had a remarkable synergistic effect (IC50 of the mixture: 0.201 mg/mL). The inhibitory mechanism of C3G-catechin mixture was of mixed type. The C3G-catechin mixture had a great impact on PL secondary structures. Molecular docking analysis further demonstrated that these polyphenols formed hydrophobic interactions and hydrogen bonds with amino acid residues in the binding pocket of PL. Collectively, C3G and catechin were shown to inhibit PL in a synergistic manner and can be potentially used for the development of food supplements for obesity prevention.
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Authors | Yuying Wang, Lihang Chen, Huimin Liu, Jiahan Xie, Wandi Yin, Zhenghang Xu, Huijing Ma, Wenzhuo Wu, Mingzhu Zheng, Meihong Liu, Jingsheng Liu |
Journal | Food chemistry
(Food Chem)
Vol. 404
Issue Pt B
Pg. 134672
(Mar 15 2023)
ISSN: 1873-7072 [Electronic] England |
PMID | 36323025
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Elsevier Ltd. All rights reserved. |
Chemical References |
- cyanidin
- Catechin
- Lipase
- Glucosides
- Anthocyanins
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Topics |
- Catechin
(pharmacology, chemistry)
- Lipase
- Molecular Docking Simulation
- Glucosides
(chemistry)
- Anthocyanins
(chemistry)
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