Inhibition of cardiomyocyte apoptosis post-acute myocardial infarction through the efficient delivery of microRNA-24 by silica nanoparticles.

Abstract

MicroRNA-24 (miR-24) is an apoptosis suppressor miRNA downregulated in cardiomyocytes after acute myocardial infarction (AMI). However, due to the lack of effective delivery strategies, the role of anti-apoptotic miR-24 in cardiomyocytes post-acute myocardial infarction remains unexplored. Here, we used a silica nanoparticle-based polyelectrolyte (polyethylenimine, PEI) delivery system to study the role of miR-24. These particles with good biocompatibility could be efficiently internalized into cells and release the loaded miR-24 into the cytoplasm. As a result, the overexpression of miR-24 resulted in the inhibition of the pro-apoptotic Bim, thereby inhibiting cardiomyocyte apoptosis in vitro. Furthermore, in vivo experiments revealed that over-expressed miR-24 additionally significantly improves ventricular remodeling and cardiac function in Sprague-Dawley (SD) rats after coronary artery ligation. In summary, our novel delivery system serves as a therapeutic miRNA formulation for cardiovascular disease treatment.

• Authors: Hong Yu, Yi Li, Ruirui Zhang, Mengchen Shen, Yuting Zhu, Qiang Zhang, Huiliang Liu, Dong Han, Xiaoli Shi, Jiao Zhang
• Journal: Nanoscale advances (Nanoscale Adv) Vol. 3 Issue 22 Pg. 6379-6385 (Nov 09 2021) ISSN: 2516-0230 [Electronic] England
• PMID: 36133483 (Publication Type: Journal Article)
• Copyright: This journal is © The Royal Society of Chemistry.