Abstract | BACKGROUND: OBJECTIVES: An unresolved question is whether conversion of soluble fibrinogen to a crosslinked fibrin matrix is required to exacerbate obesity-driven diseases. METHODS: RESULTS AND CONCLUSIONS: Consistent with prior studies, Fib390-396A mice were significantly protected from increased adiposity, NAFLD, hypercholesterolemia, and diabetes while Fib- and siFga-treated mice gained as much weight and developed obesity-associated pathologies identical to wildtype mice. FibAEK and FXIII- mice displayed an intermediate phenotype with partial protection from some obesity-associated pathologies. Results here indicate that fibrin( ogen) lacking αM β2 binding function offers substantial protection from obesity and associated disease that is partially recapitulated by preventing fibrin polymer formation or crosslinking of the wildtype molecule, but not by reduction or complete elimination of fibrinogen. Finally, these findings support the concept that fibrin polymerization and crosslinking are required for the full implementation of fibrin-driven inflammation in obesity.
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Authors | Woosuk S Hur, Katharine C King, Yesha N Patel, Y-Van Nguyen, Zimu Wei, Yi Yang, Lih Jiin Juang, Jerry Leung, Christian J Kastrup, Alisa S Wolberg, James P Luyendyk, Matthew J Flick |
Journal | Journal of thrombosis and haemostasis : JTH
(J Thromb Haemost)
Vol. 20
Issue 12
Pg. 2873-2886
(12 2022)
ISSN: 1538-7836 [Electronic] England |
PMID | 36111375
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural)
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Copyright | © 2022 International Society on Thrombosis and Haemostasis. |
Chemical References |
- Fibrin
- Polymers
- Fibrinogen
- Factor XIII
- Hemostatics
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Topics |
- Mice
- Animals
- Fibrin
(metabolism)
- Polymers
- Non-alcoholic Fatty Liver Disease
(genetics, prevention & control)
- Diabetes Mellitus, Type 2
- Afibrinogenemia
- Fibrinogen
(genetics, metabolism)
- Factor XIII
(metabolism)
- Obesity
- Hemostatics
- Diet
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