Sanhuang xiexin decoction ameliorates secondary liver injury in DSS-induced colitis involve regulating inflammation and bile acid metabolism.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

SanHuang XieXin decoction (SXD) is a widely applicated traditional Chinese medicine (TCM) with a significant gut-liver axis regulation effect.

AIM OF THE STUDY:

To evaluate the therapeutic effect and elucidate the possible underlying molecular mechanisms of SXD on liver damage secondary to ulcerative colitis (UC) in mice.

MATERIALS AND METHODS:

A model of liver damage secondary to UC was induced by drinking 5% dextran sodium sulfate (DSS) in mice. These mice were treated with one of three doses of SXD or sulfasalazine (SASP), then liver samples were collected and tested.

RESULTS:

The results reveal that SXD treatment reduced liver cells swelling, and inhibited the accumulation of the hepatic-pro-inflammatory cytokines IL-1β and tumor necrosis factor-α (TNF-α) in mice with colitis. In addition, SXD reduced the production of nitric oxide (NO) and malondialdehyde (MDA), and increased the activities of superoxide dismutase (SOD). In inflammation regulating, SXD significantly down regulated the protein expression of MyD88 and p-Iκα, but upregulated Iκα. In bile acid metabolism regulating, SXD significantly down regulated the protein expression of FXR, MRP2, BESP and SHP. Therefore, SXD treatment can regulate the TLR4-NF-κB and bile acid metabolism pathways to alleviate liver inflammation and cholestasis.

CONCLUSIONS:

These results demonstrate that SXD is a potential alternative therapeutic medicine for the treatment of liver damage secondary to colitis.

• Authors: Lixia Li, Yingjie Wang, Ling Zhao, Gang Ye, Fei Shi, Yinglun Li, Yuanfeng Zou, Xu Song, Xinghong Zhao, Zhongqiong Yin, Xueyuan Wu, Wen Li, Huaqiao Tang
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 299 Pg. 115682 (Dec 05 2022) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 36058478 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Elsevier B.V. All rights reserved.

Chemical References

• Bile Acids and Salts
• Cytokines
• Drugs, Chinese Herbal
• Myeloid Differentiation Factor 88
• NF-kappa B
• Toll-Like Receptor 4
• Tumor Necrosis Factor-alpha
• Xiexin decoction
• sanhuang
• Nitric Oxide
• Sulfasalazine
• Malondialdehyde
• Dextran Sulfate
• Superoxide Dismutase

Topics

• Animals
• Bile Acids and Salts
• Colitis (chemically induced)
• Colitis, Ulcerative (chemically induced, drug therapy)
• Cytokines (metabolism)
• Dextran Sulfate (toxicity)
• Disease Models, Animal
• Drugs, Chinese Herbal
• Inflammation (drug therapy)
• Liver (metabolism)
• Malondialdehyde
• Mice
• Myeloid Differentiation Factor 88 (metabolism)
• NF-kappa B (metabolism)
• Nitric Oxide (metabolism)
• Sulfasalazine (therapeutic use)
• Superoxide Dismutase (metabolism)
• Toll-Like Receptor 4 (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)