Abstract | BACKGROUND:
Spina bifida aperta (SBA) is a relatively common clinical type of neural tube defect. Although prenatal fetal surgery has been proven to be an effective treatment for SBA, the recovery of neurological function remains unsatisfactory due to neuron deficiencies. Our previous results demonstrated that intra-amniotic transplanted bone marrow mesenchymal stem cells (BMSCs) could preserve neural function through lesion-specific engraftment and regeneration. To further optimize the role of BMSCs and improve the environment of defective spinal cords so as to make it more conducive to nerve repair, the intra-amniotic transplanted BMSCs were modified with brain-derived neurotrophic factor ( BDNF-BMSCs), and the therapeutic potential of BDNF-BMSCs was verified in this study. METHODS: RESULTS: We found that BDNF-BMSCs sustained the characteristic of directional migration, engrafted at the SBA lesion area, increased the expression of BDNF in the defective spinal cords, alleviated the apoptosis of spinal cord cells, differentiated into neurons and skin-like cells, reduced the area of skin lesions, and improved the amniotic fluid microenvironment. Moreover, the BDNF-modified BMSCs showed a better effect than pure BMSCs on the inhibition of apoptosis and promotion of neural differentiation. CONCLUSION: These findings collectively indicate that intra-amniotic transplanted BDNF-BMSCs have an advantage of promoting the recovery of defective neural tissue of SBA fetuses.
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Authors | Wei Ma, Xiaowei Wei, Hui Gu, Dan Liu, Wenting Luo, Songying Cao, Shanshan Jia, Yiwen He, Lizhu Chen, Yuzuo Bai, Zhengwei Yuan |
Journal | Stem cell research & therapy
(Stem Cell Res Ther)
Vol. 13
Issue 1
Pg. 413
(08 13 2022)
ISSN: 1757-6512 [Electronic] England |
PMID | 35964077
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2022. The Author(s). |
Chemical References |
- Brain-Derived Neurotrophic Factor
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Topics |
- Amniotic Fluid
- Animals
- Brain-Derived Neurotrophic Factor
(genetics)
- Female
- Mesenchymal Stem Cell Transplantation
(methods)
- Mesenchymal Stem Cells
- Pregnancy
- Rats
- Spina Bifida Cystica
(chemically induced, therapy)
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