Abstract | AIMS: MATERIALS AND METHODS: Guinea pigs were sensitized and challenged by ovalbumin to induce an allergic asthma model. Then, the effects of FAAH inhibitor URB597, MAGL inhibitor JZL184, and dual (FAAH/MAGL) inhibitor JZL195 on airway inflammation and hyperreactivity were evaluated. KEY FINDINGS:
Ovalbumin challenge increased airway reactivity, IgE in serum, IL-4, and IL-13, and the percentage of eosinophils in bronchoalveolar lavage (BAL). In addition, inhibition of FAAH or MAGL enzymes leads to an increase in endocannabinoid levels. The selective inhibition of the FAAH enzyme prevented inflammation indicators such as cytokine production and inflammatory cell infiltration but had a negligible effect on airway hyperreactivity. However, the inhibition of the MAGL enzyme or dual inhibition of both FAAH and MAGL enzymes tent to moderate both pulmonary inflammation and airway hyperreactivity. SIGNIFICANCE:
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Authors | Reshed Abohalaka, Yasemin Karaman, Tuba Recber, Sevgen Celik Onder, Emirhan Nemutlu, Turgut Emrah Bozkurt |
Journal | Life sciences
(Life Sci)
Vol. 306
Pg. 120808
(Oct 01 2022)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 35843343
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Elsevier Inc. All rights reserved. |
Chemical References |
- Endocannabinoids
- Enzyme Inhibitors
- Ovalbumin
- Monoacylglycerol Lipases
- Amidohydrolases
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Topics |
- Amidohydrolases
- Animals
- Asthma
(chemically induced, drug therapy)
- Endocannabinoids
(metabolism)
- Enzyme Inhibitors
(pharmacology)
- Guinea Pigs
- Inflammation
(drug therapy)
- Mammals
(metabolism)
- Monoacylglycerol Lipases
- Ovalbumin
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