Antimicrobial-resistant is a major challenge in to treat infected
wounds, and new formulations should be produced.
Citral (Citl),
chitosan (Chsn), and
zinc oxide (ZnO) nanoparticles may accelerate the wound healing process in terms of their antibacterial properties. This new study aimed to investigate the effects of
ointments produced from ZnO/Chsn/Citl nanoparticles (NPs) to treat the infected
wounds. Following the preparation of ZnO/Chsn/Citl-NPs, swelling behavior, the release of
citral, toxicity, and antibacterial properties were evaluated. Base
ointment,
mupirocin, and
ointments made from Chsn-NPs, Chsn/Citl-NPs, and ZnO/Chsn/Citl-NPs were used to treat the mice. The
ointments' effects on
wound contraction, total bacterial count, and immunofluorescence staining for TNF-α, TGF-β, and bFGF were tested. The synthesis of ZnO/Chsn/Citl-NPs was validated by XRD, FT-IR, DLS, and TEM findings. In higher dilutions,
chitosan/
citral and ZnO/Chsn/Citl-NPs indicated better antibacterial activity. Nanoparticles were safe up to concentration of the 0.5 mg/mL. The mice in Chsn/Citl and ZnO/Chsn/Citl-NPs treated groups showed higher (P < 0.05)
wound contraction ratio and expressions for bFGF, and lower total bacterial count and expressions for TGF-β and TNF-α compared to control mice.
Ointments prepared from ZnO/Chsn/Citl-NPs could compete with the commercial
ointment of
mupirocin and can be used to treat infected
wounds after clinical studies.