Efficacy of antimicrobial photodynamic therapy on the tongue surface in the management of halitosis - A real-time polymerase chain reaction analysis.

Abstract

AIM:

To evaluate the additional effect of a single session of antimicrobial photodynamic therapy (aPDT) on the tongue as an adjunct to scaling and root planing (SRP) on most common volatile sulfur-producing microbes such as Porphyromonas gingivalis (Pg), Treponema denticola (Td) and Fusobacterium nucleatum (Fn) on 3rd, 7th and 14th day postoperatively using RT-PCR analysis.

METHOD AND MATERIALS:

Twenty-four patients of either sex, presented with moderate to severe malodor, detected by a portable breath checker (Tanita®) were considered for the study and assigned to group A & B. Scaling and root planning was performed in both the groups, followed by photodynamic therapy on the tongue surface in group A. One percent methylene blue photosensitizer was applied on the middle and posterior thirds of the dorsum of the tongue and irradiated in continuous mode at six different points for 90 s at each point.

RESULTS:

A significant reduction in Halimeter scores throughout the study period was observed. A significant reduction in the tongue coating score on 3rd and 7th day and the total count of Porphyromonas gingivalis (Pg) and Fusobacterium nucleatum (Fn) on 7th and 14th day was seen in group A (p ≤ 0.05). However, the mean reduction in Treponema denticola (Td) was non-significant in both the groups but a greater fall in the total count was seen in group A compared to group B on all the days (p ≥ 0.05).

CONCLUSION:

Within the limitations of the study, it was concluded that photodynamic therapy on the tongue along with scaling and root planing was effective in the reduction of malodour and the total count of bacteria responsible for the same. Long-term clinical trials are required to further substantiate the effectiveness of this technique.

• Authors: Pooja Patil, Leena Patil, M G Triveni, G V Usha, Rucha Shah, A B Tarun Kumar
• Journal: Photodiagnosis and photodynamic therapy (Photodiagnosis Photodyn Ther) Vol. 39 Pg. 102989 (Sep 2022) ISSN: 1873-1597 [Electronic] Netherlands
• PMID: 35792253 (Publication Type: Journal Article)
• Copyright: Copyright © 2022. Published by Elsevier B.V.

Chemical References

• Anti-Bacterial Agents
• Anti-Infective Agents

Topics

• Anti-Bacterial Agents (pharmacology)
• Anti-Infective Agents (therapeutic use)
• Fusobacterium nucleatum
• Halitosis (drug therapy, microbiology)
• Humans
• Photochemotherapy (methods)
• Porphyromonas gingivalis
• Real-Time Polymerase Chain Reaction
• Tongue (microbiology)
• Treponema denticola