Abstract |
The impact of low-dose spironolactone (LSPL) on polycystic ovarian syndrome (PCOS)-associated cardio-renal disorder is unknown. Therefore, the present study hypothesized that LSPL would ameliorate cardio-renal disorders in experimental PCOS animals. Eight-week-old female Wistar rats were allotted into three groups. The control group received vehicle (distilled water; per os (p.o.)), the letrozole (LET)-treated group designated as PCOS group received LET (1 mg/kg; p.o.), and PCOS+LSPL received LET and LSPL (0.25 mg/kg, p.o.). The treatment was done once daily for 21 days uninterrupted. The experimental PCOS rats were characterized with insulin resistance, as well as elevated testosterone and luteinizing hormone/ follicle-stimulating hormone, with a significant increase in cardiac and renal lipid profile, oxidative stress, inflammatory biomarkers (nuclear factor-κB and tumor necrosis factor-α), lactate dehydrogenase and lactate content and decrease in cardiac and renal antioxidant system ( glutathione peroxidase and reduced glutathione) compared with the control rats. In addition, immunohistochemical assessment of cardiac and renal tissue showed significant expression of inflammasome and B-cell lymphoma-2 associated X- protein (BAX) in animals with PCOS. Nevertheless, these perturbations were attenuated following the administration of LSPL. Collectively, the present results suggest that LSPL attenuates PCOS-associated cardio-renal disorders by reduction of oxidative stress and BAX/ inflammasome expression.
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Authors | Mmenyene U Peter, Stephanie E Areloegbe, Christopher O Akintayo, Adesola A Oniyide, Ayodeji Aturamu, Kehinde S Olaniyi |
Journal | Canadian journal of physiology and pharmacology
(Can J Physiol Pharmacol)
Vol. 100
Issue 9
Pg. 890-902
(Sep 01 2022)
ISSN: 1205-7541 [Electronic] Canada |
PMID | 35771488
(Publication Type: Journal Article)
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Chemical References |
- Inflammasomes
- bcl-2-Associated X Protein
- Spironolactone
- Letrozole
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Topics |
- Animals
- Disease Models, Animal
- Female
- Humans
- Inflammasomes
- Letrozole
(therapeutic use)
- Polycystic Ovary Syndrome
(drug therapy, metabolism)
- Rats
- Rats, Wistar
- Spironolactone
(pharmacology, therapeutic use)
- bcl-2-Associated X Protein
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