Adverse effects of SYP-3343 on zebrafish development via ROS-mediated mitochondrial dysfunction.

Abstract

As a newly-invented and highly-efficiency strobilurin fungicide, pyraoxystrobin (SYP-3343) has been recognized as a highly poisonous toxin for a variety of aquatic organisms. Nevertheless, the developmental toxicity and potential mechanism of SYP-3343 have not been well-documented. The results showed that SYP-3343 was relatively stable and maintained within the range of 20 % in 24 h, and the LC50 value to embryos at 72 hpf was 17.13 μg/L. The zebrafish embryotoxicity induced by 1, 2, 4, and 8 μg/L SYP-3343 is demonstrated by repressive embryo incubation, enhancive mortality rate, abnormal heart rate, malformed morphological characteristic, and impaired spontaneous coiling, indicating SYP-3343 mostly exerted its toxicity in a dose- and time-dependent manner. Besides SYP-3343 was critically involved in regulating cell cycle, mitochondrial membrane potential, and reactive oxygen species production as well as zebrafish primary cells apoptosis, which can be mitigated using antioxidant N-acetyl-L-cysteine. A significant change occurred in total protein content, the biochemical indices, and antioxidant capacities owing to SYP-3343 exposure. Additionally, SYP-3343 altered the mRNA levels of heart development-, mitochondrial function-, and apoptosis-related genes in zebrafish embryos. These results indicated that SYP-3343 induced apoptosis accompanying reactive oxygen species-initiated mitochondrial dysfunction in zebrafish embryos.

• Authors: Guoliang Chen, Mingxing Wang, Panpan Zhu, Guixue Wang, Tingzhang Hu
• Journal: Journal of hazardous materials (J Hazard Mater) Vol. 437 Pg. 129382 (09 05 2022) ISSN: 1873-3336 [Electronic] Netherlands
• PMID: 35749898 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 Elsevier B.V. All rights reserved.

Chemical References

• Antioxidants
• Reactive Oxygen Species

Topics

• Animals
• Antioxidants (metabolism)
• Apoptosis
• Embryo, Nonmammalian
• Mitochondria
• Oxidative Stress
• Reactive Oxygen Species (metabolism)
• Zebrafish (metabolism)