Abstract |
Bronchial asthma is characterized by chronic airway inflammation, airway hyperresponsiveness, and airway remodeling. MicroRNA ( miRNA) has recently been implicated in the pathogenesis of asthma. However, the mechanisms of different miRNAs in asthma are complicated, and the mechanism of miRNA-182-5p in asthma is still unclear. Here, we aim to explore the mechanism of miRNA182-5p in asthma-related airway inflammation. Ovalbumin (OVA)-induced asthma model was established. MiRNA Microarray Analysis was performed to analyze the differentially expressed miRNAs in the asthma model. We found that the expression of miRNA-182-5p was significantly decreased in OVA-induced asthma. In vitro, IL-13 stimulation of BEAS-2B cells resulted in a significant up-regulation of NOX4 ( nicotinamide adenine dinucleotide phosphate oxidase 4), accompanied by mitochondrial damage-induced apoptosis, NLRP3 ( NOD-like receptor family pyrin domain-containing 3)/IL-1β activation, and reduced miRNA-182-5p. In contrast, overexpression of miRNA-182-5p significantly inhibited epithelial cell apoptosis and NLRP3/IL-1β activation. In addition, we found that miRNA-182-5p could bind to the 3' untranscripted region of NOX4 mRNA and inhibit epithelial cell inflammation by reducing oxidative stress and mitochondrial damage. In vivo, miRNA-182-5p agomir treatment significantly reduced the percentage of eosinophils in bronchoalveolar lavage fluid, and down-regulated Th2 inflammatory factors, including IL-4, IL-5, and OVA induced IL-13. Meanwhile, miRNA-182-5p agomir reduced the peribronchial inflammatory cell infiltration, goblet cell proliferation and collagen deposition. In summary, targeting miRNA-182-5p may provide a new strategy for the treatment of asthma.
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Authors | Zhiguang Wang, Yilan Song, Jingzhi Jiang, Yihua Piao, Li Li, Qiaoyun Bai, Chang Xu, Hanye Liu, Liangchang Li, Hongmei Piao, Guanghai Yan |
Journal | Frontiers in immunology
(Front Immunol)
Vol. 13
Pg. 853848
( 2022)
ISSN: 1664-3224 [Electronic] Switzerland |
PMID | 35711428
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2022 Wang, Song, Jiang, Piao, Li, Bai, Xu, Liu, Li, Piao and Yan. |
Chemical References |
- Interleukin-13
- MicroRNAs
- Mirn182 microRNA, mouse
- NLR Family, Pyrin Domain-Containing 3 Protein
- Ovalbumin
- NADPH Oxidase 4
- Nox4 protein, mouse
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Topics |
- Animals
- Asthma
(metabolism)
- Inflammation
(genetics, metabolism)
- Interleukin-13
(genetics)
- Mice
- Mice, Inbred BALB C
- MicroRNAs
(genetics)
- NADPH Oxidase 4
(genetics)
- NLR Family, Pyrin Domain-Containing 3 Protein
(genetics)
- Ovalbumin
(adverse effects)
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