Abstract | PURPOSE: METHODS: Subjects homozygotes for the TT and CC genotypes of the FADS1-rs174550 (TT, n = 25 and CC, n = 28) or -rs174547 (TT, n = 42 and CC, n = 28), were either recruited from the METabolic Syndrome In Men cohort to participate in an intervention with LA-enriched diet (FADSDIET) or from the Kuopio Obesity Surgery (KOBS) study. GC and LC-MS for plasma FA proportions and eicosanoid concentrations and AT gene expression for AT inflammatory score (AT-InSc) was determined. RESULTS: We observed a diet-genotype interaction between LA-enriched diet and AT-InSc in the FADSDIET. In the KOBS study, interleukin (IL)1 beta mRNA expression in AT was increased in subjects with the TT genotype and highest LA proportion. In the FADSDIET, n-6/LA proportions correlated positively with AT-InSc in those with the TT genotype but not with the CC genotype after LA-enriched diet. Specifically, LA- and AA-derived pro-inflammatory eicosanoids related to CYP450/sEH-pathways correlated positively with AT-InSc in those with the TT genotype, whereas in those with the CC genotype, the negative correlations between pro-inflammatory eicosanoids and AT-InSc related to COX/LOX-pathways. CONCLUSIONS: LA-enriched diet increases inflammatory AT gene expression in subjects with the TT genotype, while CC genotype could play a protective role against LA-induced AT inflammation. Overall, the FADS1 variant could modify the dietary LA-induced effects on AT inflammation through the differential biosynthesis of AA-derived eicosanoids.
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Authors | Maija Vaittinen, Maria A Lankinen, Pirjo Käkelä, Jyrki Ågren, Craig E Wheelock, Markku Laakso, Ursula Schwab, Jussi Pihlajamäki |
Journal | European journal of nutrition
(Eur J Nutr)
Vol. 61
Issue 7
Pg. 3707-3718
(Oct 2022)
ISSN: 1436-6215 [Electronic] Germany |
PMID | 35701670
(Publication Type: Journal Article)
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Copyright | © 2022. The Author(s). |
Chemical References |
- Delta-5 Fatty Acid Desaturase
- Eicosanoids
- Linoleic Acid
- FADS1 protein, human
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Topics |
- Adipose Tissue
(metabolism, pathology)
- Delta-5 Fatty Acid Desaturase
(genetics)
- Diet
- Eicosanoids
(metabolism)
- Female
- Genotype
- Humans
- Inflammation
(metabolism)
- Linoleic Acid
(administration & dosage, metabolism)
- Male
- Polymorphism, Single Nucleotide
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