Kinase Inhibition by PKC412 Prevents Epithelial Sheet Damage in Autosomal Dominant Epidermolysis Bullosa Simplex through Keratin and Cell Contact Stabilization.

Abstract

Epidermolysis bullosa simplex (EBS) is a severe and potentially life-threatening disorder for which no adequate therapy exists. Most cases are caused by dominant sequence variations in keratin genes K5 or K14, leading to the formation of cytoplasmic keratin aggregates, profound keratinocyte fragility, and cytolysis. We hypothesized that pharmacological reduction of keratin aggregates, which compromise keratinocyte integrity, represents a viable strategy for the treatment of EBS. In this study, we show that the multikinase inhibitor PKC412, which is currently in clinical use for acute myeloid leukemia and advanced systemic mastocytosis, reduced keratin aggregation by 40% in patient-derived K14.R125C EBS-associated keratinocytes. Using a combination of epithelial shear stress assay and real-time impedance spectroscopy, we show that PKC412 restored intercellular adhesion. Molecularly, global phosphoproteomic analysis together with immunoblots using phosphoepitope-specific antibodies revealed that PKC412 treatment altered phosphorylated sites on keratins and desmoplakin. Thus, our data provide a proof of concept to repurpose existing drugs for the targeted treatment of EBS and showcase how one broad-range kinase inhibitor reduced keratin filament aggregation in patient-derived EBS keratinocytes and the fragility of EBS cell monolayers. Our study paves the way for a clinical trial using PKC412 for systemic or local application in patients with EBS.

• Authors: Katrin Rietscher, Heinz-Georg Jahnke, Matthias Rübsam, Eric W Lin, Cristina Has, M Bishr Omary, Carien M Niessen, Thomas M Magin
• Journal: The Journal of investigative dermatology (J Invest Dermatol) Vol. 142 Issue 12 Pg. 3282-3293 (12 2022) ISSN: 1523-1747 [Electronic] United States
• PMID: 35691363 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• Keratins
• midostaurin
• Staurosporine
• Cytoskeletal Proteins
• Keratin-14
• Keratin-5

Topics

• Humans
• Epidermolysis Bullosa Simplex (genetics, metabolism)
• Keratins (metabolism)
• Staurosporine (metabolism)
• Cytoskeleton (metabolism)
• Cytoskeletal Proteins (genetics)
• Keratin-14 (genetics, metabolism)
• Keratin-5 (genetics, metabolism)
• Mutation