Identification and Visualization of Protein Expression in Whole Mouse Embryos by Immunofluorescence.

Abstract

Mouse embryo studies are pivotal for the understanding of early development. Analysis of the spatial and temporal changes of protein expression during development of a mouse embryo allows us to identify the genetic basis of errors of development in animal disease models. Immunofluorescence is a powerful technique to study the localization and variation in expression pattern of specific proteins in cells, tissues, and organs. Detecting the antigens with their specific antibodies labeled with fluorescent probes allows visualization of proteins at the cellular level. Here, we provide the optimized protocol of immunostaining whole mouse embryos at embryonic stages E7.5 to E11.5.

• Authors: V Pragathi Masamsetti, Patrick P L Tam
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 2490 Pg. 39-45 ( 2022) ISSN: 1940-6029 [Electronic] United States
• PMID: 35486237 (Publication Type: Journal Article)
• Copyright: © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Chemical References

• Fluorescent Dyes

Topics

• Animals
• Embryo, Mammalian (metabolism)
• Fluorescent Antibody Technique
• Fluorescent Dyes (metabolism)
• Mice
• Staining and Labeling