Abstract | Background: Methods: Primary human conjunctival fibroblasts (HCFs) and human pterygium fibroblasts (HPFs) were separated and cultured from fresh conjunctiva grafts and pterygium tissues. The PLKO.1 lentiviral system and CRISPR/Cas9 recombinant construct were, respectively, used to overexpress and silence U3 snoRNA in HPFs and HCFs for further specific phenotype analysis. RNA-seq and TMT-labeled quantitative protein mass spectrometry were utilized to evaluate the effect of U3 snoRNA on mRNA transcripts and protein synthesis. Results: Reduced U3 snoRNA in pterygium promotes HCF or HPF cells' proliferation, migration, and cell cycle but has no significant effect on apoptosis. U3 snoRNA modulates 18S rRNA synthesis through shearing precursor ribosomal RNA 47S rRNA at the 5' external transcribed spacer (5' ETS). Moreover, the altered U3 snoRNA causes mRNA and protein differential expression in HCF or HPF cells. Conclusions:
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Authors | Xin Zhang, Yaping Jiang, Qian Wang, Weishu An, Xiaoyan Zhang, Ming Xu, Yihui Chen |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 63
Issue 4
Pg. 17
(04 01 2022)
ISSN: 1552-5783 [Electronic] United States |
PMID | 35472218
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA Precursors
- RNA, Messenger
- RNA, Ribosomal
- RNA, Ribosomal, 18S
- RNA, Small Nucleolar
- RNA, U3 small nucleolar
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Topics |
- Base Sequence
- Conjunctiva
(abnormalities, metabolism)
- Humans
- Pterygium
(genetics)
- RNA Precursors
(chemistry, genetics, metabolism)
- RNA Processing, Post-Transcriptional
- RNA, Messenger
(genetics, metabolism)
- RNA, Ribosomal
- RNA, Ribosomal, 18S
(chemistry, genetics, metabolism)
- RNA, Small Nucleolar
(chemistry, genetics, metabolism)
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