As a
traditional Chinese medicine, Dalbergia tsoi Merr.et Chun (JZX) has been used for the treatment of
wounds since ancient times. However, the active compounds and molecular mechanisms of JZX in the acceleration of wound healing are still unknown. Herein, we explored the main active compounds and key molecular mechanisms by which JZX accelerates wound healing. The
ethanol extract of JZX was subjected to UPLC-Q-Orbitrap HRMS analysis to identify the main compounds. The pharmacological effect of JZX on wound healing was evaluated using a mouse excision
wound model. Network pharmacology was utilized to predict the effective compounds and related signal transduction pathways of JZX that were involved in accelerating wound healing. The predicted key signaling pathways were then validated by immunohistochemical analysis. Interactions between the active compounds and therapeutic targets were confirmed by molecular docking analysis. JZX accelerated wound healing, improved tissue quality, and inhibited
inflammation and oxidative stress. Moreover, our results suggested that the active components of JZX, such as
butin, eriodyctiol, and
formononetin, are the key compounds that facilitate
wound treatment. Our studies also indicated that JZX accelerated wound healing by regulating the PI3K/Akt signaling pathway and inducing the expression of TGF-β1,
FGF2, VEGFA, ECM1, and α-SMA at different stages of skin wound healing. The JZX extract accelerates wound healing by reducing
inflammation and inhibiting oxidative stress, regulating the PI3K/Akt signaling pathway, and promoting the expression of
growth factors, suggesting that JZX has potential clinical applicability in
wound treatment.