Retinoblastoma patient-derived stem cells-an in vivo model to study the role of RB1 in adipogenesis.

Abstract

Retinoblastoma (RB1) protein is a multifunctional protein that plays an important role in cell cycle regulation and cell differentiation, including adipogenesis. A detailed literature search to understand the role of RB1 in adipogenesis revealed that the nature of the RB1 inactivation (in vivo/in vitro) led to differences in adipogenesis. The majority of these studies were animal-based, and the only study in humans employed an in vitro mode of RB1 inactivation. To overcome these differences and lack of human studies, we sought to explore the role of RB1 in adipogenesis using orbital adipose mesenchymal stem cells (OAMSCs) from patients with retinoblastoma that innately carry a heterozygous RB1 mutation. We hypothesized that these patient-derived RB1 mutant OAMSCs can model in vivo RB1 inactivation in humans. Our study revealed increased adipogenesis with a bias toward brown adipogenesis in the RB1 mutant in addition to an increased number of adipocytes in the mitotic phase.

• Authors: Ambily Vincent, Viswanathan Natarajan, Vikas Khetan, Subramanian Krishnakumar, Sowmya Parameswaran
• Journal: Histochemistry and cell biology (Histochem Cell Biol) Vol. 158 Issue 2 Pg. 181-192 (Aug 2022) ISSN: 1432-119X [Electronic] Germany
• PMID: 35445864 (Publication Type: Journal Article)
• Copyright: © 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Chemical References

• RB1 protein, human
• Retinoblastoma Binding Proteins
• Ubiquitin-Protein Ligases

Topics

• Adipocytes
• Adipogenesis (genetics)
• Cell Differentiation
• Humans
• Retinoblastoma (genetics)
• Retinoblastoma Binding Proteins (genetics)
• Stem Cells (cytology, metabolism)
• Ubiquitin-Protein Ligases (genetics)