A novel plant lectin, NTL-125, interferes with SARS-CoV-2 interaction with hACE2.

Abstract

COVID-19 caused by SARS-CoV-2 virus has had profound impact on the world in the past two years. Intense research is going on to find effective drugs to combat the disease. Over the past year several vaccines were approved for immunization. But SARS-CoV-2 being an RNA virus is continuously mutating to generate new variants, some of which develop features of immune escape. This raised serious doubts over the long-term efficacy of the vaccines. We have identified a unique mannose binding plant lectin from Narcissus tazetta bulb, NTL-125, which effectively inhibits SARS-CoV-2 replication in Vero-E6 cell line. In silico docking studies revealed that NTL-125 has strong affinity to viral Spike RBD protein, preventing it from attaching to hACE2 receptor, the gateway to cellular entry. Binding analyses revealed that all the mutant variants of Spike protein also have stronger affinity for NTL-125 than hACE2. The unique α-helical tail of NTL-125 plays most important role in binding to RBD of Spike. NTL-125 also interacts effectively with some glycan moieties of S-protein in addition to amino acid residues adding to the binding strength. Thus, NTL-125 is a highly potential antiviral compound of natural origin against SARS-CoV-2 and may serve as an important therapeutic for management of COVID-19.

• Authors: Anindya Sarkar, Sathi Paul, Charandeep Singh, Nilkanta Chowdhury, Papri Nag, Swarnava Das, Sahil Kumar, Anshul Sharma, Deepjyoti Kumar Das, Dipak Dutta, Krishan Gopal Thakur, Angshuman Bagchi, Surbhi Shriti, Kali P Das, Rajesh P Ringe, Sampa Das
• Journal: Virus research (Virus Res) Vol. 315 Pg. 198768 (07 02 2022) ISSN: 1872-7492 [Electronic] Netherlands
• PMID: 35398454 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022. Published by Elsevier B.V.

Chemical References

• Plant Lectins
• Spike Glycoprotein, Coronavirus
• spike protein, SARS-CoV-2
• ACE2 protein, human
• Angiotensin-Converting Enzyme 2

Topics

• Angiotensin-Converting Enzyme 2
• COVID-19
• Humans
• Narcissus (chemistry)
• Plant Lectins (pharmacology)
• Protein Binding
• SARS-CoV-2 (drug effects)
• Spike Glycoprotein, Coronavirus (antagonists & inhibitors, chemistry)