High-Resolution Mass Spectrometry for the Measurement of PTH and PTH Fragments: Insights into PTH Physiology and Bioactivity.

Abstract

Full-length parathyroid hormone (PTH 1-84) is crucial for the regulation of calcium and phosphate homeostasis and bone remodeling. PTH 1-84 is metabolized into various PTH fragments, which are measured with varying levels of efficiency by PTH immunoassays. These PTH fragments, which increase in serum as CKD progresses, could potentially modulate the effects of PTH 1-84 and contribute to CKD-associated bone disorders. To obtain a true biologic representation of total PTH bioactivity, it is necessary to measure not only PTH 1-84 but also PTH fragments that are present in circulation. Traditional second-generation PTH immunoassays collectively measure PTH 1-84, PTH fragments, and post-translationally modified PTH 1-84, making it difficult to accurately predict the character of underlying renal osteodystrophy. This review highlights current advances in methods available for PTH measurement and the clinical relevance of PTH fragments in CKD. We emphasize the usefulness of mass spectrometry as a potential reference method for PTH measurement.

• Authors: Candice Z Ulmer, Kittrawee Kritmetapak, Ravinder J Singh, Hubert W Vesper, Rajiv Kumar
• Journal: Journal of the American Society of Nephrology : JASN (J Am Soc Nephrol) Vol. 33 Issue 8 Pg. 1448-1458 (08 2022) ISSN: 1533-3450 [Electronic] United States
• PMID: 35396262 (Publication Type: Journal Article, Review, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 by the American Society of Nephrology.

Chemical References

• Parathyroid Hormone
• Peptide Fragments

Topics

• Bone and Bones
• Chronic Kidney Disease-Mineral and Bone Disorder
• Humans
• Mass Spectrometry
• Parathyroid Hormone
• Peptide Fragments
• Renal Insufficiency, Chronic