Abstract |
As bone marrow transplant (BMT) is gradually applied to the study of central nervous system ( CNS) disease, it is needed to investigate the proper dose of chemotherapy to eradicate bone marrow cells while bringing little damage to brain. In the present study, we established a BMT model with varied busulfan and cyclophosphamide (Bu-Cy) dosages. The recipient mice's chimera rate, neuronal death, neuroinflammation, and behavioral functions were all investigated. Chimerism of peripheral blood cells was shown to rise with Bu-Cy treatment doses, with 60.7% in the Bu(20 mg/kg)/Cy(100 mg/kg) group and 93.0% in the Bu(35 mg/kg)/Cy(100 mg/kg) group. Recipients with Bu(35 mg/kg)/Cy(100 mg/kg) therapy had brain injury, increased neuroinflammation, diminished neurogenesis and cognitive abnormalities, whereas animals given a lesser dosage had no such brain damages. Conclusively, considering the chimerism and the possibility to damage brain, we recommend Bu(20 mg/kg)/Cy(100 mg/kg) is the ideal dose in BMT for studying CNS diseases in the C57/BL6 mouse strain.
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Authors | Zhong-Yuan Yu, Man-Yu Xu, Zhi-Hao Liu, Gui-Hua Zeng, Huan Fan, Cheng-Rong Tan, Yun-Feng Tu, Xian-Le Bu, Yan-Jiang Wang |
Journal | Neurotoxicity research
(Neurotox Res)
Vol. 40
Issue 2
Pg. 585-595
(Apr 2022)
ISSN: 1476-3524 [Electronic] United States |
PMID | 35380369
(Publication Type: Journal Article)
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Copyright | © 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. |
Chemical References |
- Cyclophosphamide
- Busulfan
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Topics |
- Animals
- Bone Marrow Transplantation
- Busulfan
(therapeutic use, toxicity)
- Cyclophosphamide
(toxicity)
- Mice
- Neurogenesis
- Neuroinflammatory Diseases
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