Dufulin is a biologically derived
antiviral agent chemically synthesized by α-
phosphoramidate in sheep and is effective against
viral diseases in plants such as tobacco, rice, cucumber and tomato. However, the environmental behaviors and fate of
Dufulin under different cultivation systems remain unknown. This study investigates the absorption, translocation and accumulation of 14C-Dufulin stereoisomers introduced by
pesticide leaf daubing and by mixing the
pesticide with soil in different tissues of cherry radish. We particularly focused on whether the behaviors of
Dufulin enantiomers in plants were stereoselective. In the leaf uptake experiments, S-
Dufulin and R-
Dufulin were transported both up and down, while more than 93% of the
pesticide remained in the labeled leaves. During the radicular absorption experiments, both enantiomers of
Dufulin were taken up by radish roots and moved to the upper part of the plant, while less than 0.2%
Dufulin was absorbed from the soil. Hence, it was easier for
Dufulin to enter plants through the leaf surface than through the roots. However, we found in this trial that the stereoisomers of
Dufulin underwent nonstereoselective absorption and translocation, which implies that rac-
Dufulin and its metabolites should be a major research priority. Overall, our results provide a relatively accurate prediction of the risk assessment of
Dufulin, which will help guide its rational use in the environment as well as ensure eco-environmental safety and human health.