Immunohistochemical algorithms and gene expression profiling in primary cutaneous B-cell lymphoma.

Abstract

OBJECTIVE:

to assess whether immunohistochemical (IHC) algorithms used to classify the cell of origin (COO) of nodal Diffuse Large B-cell lymphoma (nDLBCL) in Germinal Center type (GCB) and non-GCB subtypes may be applied to Primary Cutaneous B-cell lymphoma (PCBCL) too, and which of these algorithms performs better on PCBCL.

DESIGN:

Retrospective case control study.

SETTING:

Pathology Department of the University Hospital "San Giovanni di Dio e Ruggi d'Aragona" Salerno, Italy.

PARTICIPANTS:

Fourteen PCBCL, including Primary Cutaneous follicle centre lymphoma (PCFCL) and primary cutaneous diffuse large B-cell lymphoma, Leg type (PCDLBCL-LT) and 14 nDLBCL were evaluated for 7-year period (January 2011 to December 2017). Primary cutaneous marginal zone cell lymphoma (PCMZL) cases were not included in the present study.

INTERVENTION:

Evaluation of immunohistochemical CD10, BCL6, MUM1/IRF4, BCL2, MYC and Ki-67 expression and classification according to three different algorithms. Gene expression profiling (GEP) was performed on the same series using Lymph2Cx assay (Nanostring). The data obtained were compared and analysed.

RESULTS:

All the IHC algorithms showed 13 GCB and 15 non-GCB. GEP showed 12 GCB, 12 activated B cell-type and 4 unclassified.

CONCLUSIONS:

The PCBCL were classifiable as GCB and non-GCB like the nDLBCL as IHC algorithms were concordant to GEP and produced the same results.

• Authors: Pasquale Cretella, Anna Lucia Peluso, Caterina Picariello, Immacolata Cozzolino, Massimo Triggiani, Alessandro Puzziello, Valentina Giudice, Francesco Sabbatino, Antonio Ieni, Pio Zeppa, Alessandro Caputo
• Journal: Pathology, research and practice (Pathol Res Pract) Vol. 231 Pg. 153804 (Mar 2022) ISSN: 1618-0631 [Electronic] Germany
• PMID: 35183824 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Elsevier GmbH. All rights reserved.

Chemical References

• Biomarkers, Tumor

Topics

• Aged
• Aged, 80 and over
• Algorithms
• Biomarkers, Tumor (analysis, blood)
• Case-Control Studies
• Female
• Gene Expression (genetics, physiology)
• Humans
• Immunohistochemistry (methods, statistics & numerical data)
• Italy (epidemiology)
• Lymphoma, B-Cell (diagnosis, epidemiology, genetics)
• Male
• Middle Aged
• Retrospective Studies