Abstract | INTRODUCTION: MATERIALS AND METHODS: The study enrolled 160 breast cancer patients and 160 healthy control females. LEPR Q223R polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum leptin was determined using enzyme-linked immunosorbent assay human leptin kit. Immunohistochemical tests from paraffin blocks were carried out for estrogen and progesterone staging using the precise antibodies. RESULTS: An association was found between LEPR gene Q223R gene polymorphism among obese breast cancer females. Statistical difference was found between GG (60.6%) Arg/Arg genotype (OR=2.986; 95%CI=1.540 to 5.789; p= 0.001) compared to AA (33.1%) Gln/Gln genotype. GG Q223R LEPR polymorphism showed statistically significant difference among obese breast cancer patients (BMI more than 25) compared to control (P < 0.0001). GG genotype of Q223R LEPR polymorphism showed statistically significant increased leptin level (p-value =0.0001) among obese patients (mean± SD; 23.39±4.32) compared to control (17.83±5.67). CONCLUSIONS: Q223R LEPR polymorphism GG genotype was associated with increased leptin profile among obese breast cancer females.
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Authors | Manar F Atoum, Ala'a Abdel Hamaid Alparrey |
Journal | Asian Pacific journal of cancer prevention : APJCP
(Asian Pac J Cancer Prev)
Vol. 23
Issue 1
Pg. 177-182
(Jan 01 2022)
ISSN: 2476-762X [Electronic] Thailand |
PMID | 35092386
(Publication Type: Journal Article)
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Chemical References |
- LEP protein, human
- LEPR protein, human
- Leptin
- Receptors, Leptin
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Topics |
- Breast Neoplasms
(blood, genetics)
- Case-Control Studies
- Female
- Genetic Predisposition to Disease
(genetics)
- Genotype
- Humans
- Leptin
(blood)
- Middle Aged
- Obesity
(blood, genetics)
- Polymorphism, Restriction Fragment Length
(genetics)
- Receptors, Leptin
(genetics)
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