Serum Metabolomics in Patients with Coexisting NAFLD and T2DM Using Liquid Chromatography-Mass Spectrometry.

Abstract

OBJECTIVE:

Nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM) frequently coexist and can act synergistically to drive adverse outcomes of one another. This study aimed to unravel the metabolomic changes in patients with NAFLD and T2DM, to identify potential noninvasive biomarkers, and to provide insights for understanding the link between NAFLD and T2DM.

METHODS:

Three hundred participants aged 35 to 70 years who were diagnosed with NAFLD (n = 100), T2DM (n = 100), or a comorbidity of NAFLD and T2DM (n = 100) were included in this study. Anthropometrics and routine blood chemistry were assessed after overnight fast. The global serum metabolomic analysis was performed by ultra-performance liquid chromatography-Orbitrap mass spectrometry. Multivariate data analysis methods were utilized to identify the potential biomarkers.

RESULTS:

A set of serum biomarkers that could effectively separate NAFLD from NAFLD + T2DM and T2DM from NAFLD + T2DM were identified. We found that patients with coexisting NAFLD and T2DM had significantly higher levels of total protein (TP), triglycerides (TG), glucose in urine, and gamma-hydroxybutyric acid than those with NAFLD and had significant increased levels of TP, albumin, alanine aminotransferase, aspartate aminotransferase, total cholesterol, cholinesterase, TG, low-density lipoprotein, and apolipoprotein A when compared to patients with T2DM.

CONCLUSION:

The metabolomics results provide evidence that the comorbidity of NAFLD and T2DM considerably altered patients' metabolomics patterns compared to those of patients with only NAFLD or T2DM.

• Authors: Cheng Hu, Xiaoyu Zhuang, Jiaqi Zhang, Tao Wang, Shengnan Du, Jinping Wang, Xuelian Peng, Qin Cao, Mingcai Zhang, Yuanye Jiang
• Journal: Laboratory medicine (Lab Med) Vol. 53 Issue 4 Pg. 360-368 (Jul 04 2022) ISSN: 1943-7730 [Electronic] England
• PMID: 35075477 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2022. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: [email protected].

Chemical References

• Biomarkers

Topics

• Biomarkers
• Chromatography, Liquid
• Diabetes Mellitus, Type 2 (complications, epidemiology)
• Humans
• Mass Spectrometry
• Metabolomics (methods)
• Non-alcoholic Fatty Liver Disease (complications, epidemiology)