Abstract |
The epithelial cell rests of Malassez (ERM) are essential in preventing ankylosis between the alveolar bone and the tooth ( dentoalveolar ankylosis). Despite extensive research, the mechanism by which ERM cells suppress ankylosis remains uncertain; perhaps its varied population is to reason. Therefore, in this study, eighteen unique clones of ERM (CRUDE) were isolated using the single-cell limiting dilution and designated as ERM 1-18. qRT-PCR, ELISA, and western blot analyses revealed that ERM-2 and -3 had the highest and lowest amelogenin expression, respectively. Mineralization of human periodontal ligament fibroblasts (HPDLF) was reduced in vitro co-culture with CRUDE ERM, ERM-2, and -3 cells, but recovered when an anti- amelogenin antibody was introduced. Transplanted rat molars grown in ERM-2 cell supernatants produced substantially less bone than those cultured in other cell supernatants; inhibition was rescued when an anti- amelogenin antibody was added to the supernatants. Anti-Osterix antibody staining was used to confirm the development of new bones. In addition, next-generation sequencing (NGS) data were analysed to discover genes related to the distinct roles of CRUDE ERM, ERM-2, and ERM-3. According to this study, amelogenin produced by ERM cells helps to prevent dentoalveolar ankylosis and maintain periodontal ligament (PDL) space, depending on their clonal diversity.
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Authors | Syed Taufiqul Islam, Yoshihito Kurashige, Erika Minowa, Koki Yoshida, Durga Paudel, Osamu Uehara, Yunosuke Okada, Dembereldorj Bolortsetseg, Sayaka Sakakibara, Yoshihiro Abiko, Masato Saitoh |
Journal | Scientific reports
(Sci Rep)
Vol. 12
Issue 1
Pg. 382
(01 10 2022)
ISSN: 2045-2322 [Electronic] England |
PMID | 35013397
(Publication Type: Journal Article)
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Copyright | © 2022. The Author(s). |
Chemical References |
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Topics |
- Amelogenin
(genetics, metabolism)
- Animals
- Cell Proliferation
- Cell Separation
- Cells, Cultured
- Coculture Techniques
- Disease Models, Animal
- Epithelial Cells
(metabolism, pathology)
- Fibroblasts
(metabolism, pathology)
- Gene Expression Regulation
- Humans
- Male
- Molar
(metabolism, pathology, transplantation)
- Osteogenesis
- Periodontal Ligament
(metabolism, pathology)
- Phenotype
- Rats, Wistar
- Sus scrofa
- Tooth Ankylosis
(genetics, metabolism, pathology, prevention & control)
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