Evaluation of the protective effects of gossypin for ischemia/reperfusion injury in ovary tissue.

Abstract

AIM:

Ovarian ischemia-reperfusion (I/R) injury is a serious gynecological condition that affects women of reproductive age and reduces ovarian reserve. Management of I/R injury with detorsion causes reperfusion damage, in which oxidative stress plays a central role. This study aimed to investigate whether the gossypin (GOS) with antioxidant properties, a flavonoid, has beneficial effects on the biochemical, molecular, and histopathological aspects of ovarian I/R injury.

METHODS:

Thirty-three female Balb/c mice were randomly divided into five groups as follows: Healthy (Sham-operated control group), I/R (IR group), I/R + GOS 5 (I/R with GOS 5 mg/kg), I/R + GOS 10 (I/R with GOS 10 mg/kg), and I/R + GOS 20 (I/R with GOS 20 mg/kg). This was followed by 3 h of ischemia and subsequent reperfusion for 3 h after detorsion was exposed. GOS was injected 2 h before reperfusion.

RESULTS:

IL-1β, IL-6, TNF-α, NF-κB, and CASP-3 mRNA expressions, SOD (superoxide dismutase) activity, GSH (glutathione), and MDA (malondialdehyde) levels, and histopathological changes were evaluated in ovarian tissue. Histological examination indicated that treatment of ovarian I/R injury with GOS led to the improvement of ovarian tissue, which was accompanied by an increase in SOD activity and GSH level and a decrease in MDA level, NF-κB, TNF-α, IL-1β, and IL-6 expressions. GOS was also corrected by reducing the elevated expression of CASP-3 as apoptosis-change marker.

CONCLUSION:

These findings indicate that the treatment of GOS may be useful as a conservative approach to reverse I/R injury via amelioration of oxidative stress parameters and histopathological scores, attenuation of inflammation, and the suppression of apoptosis.

• Authors: Busra Dincer, Irfan Cinar, Muhammed Yayla, Erdem Toktay
• Journal: The journal of obstetrics and gynaecology research (J Obstet Gynaecol Res) Vol. 48 Issue 3 Pg. 748-756 (Mar 2022) ISSN: 1447-0756 [Electronic] Australia
• PMID: 34931736 (Publication Type: Journal Article)
• Copyright: © 2021 Japan Society of Obstetrics and Gynecology.

Chemical References

• Antioxidants
• Flavonoids
• Malondialdehyde
• gossypin

Topics

• Animals
• Antioxidants (metabolism, pharmacology)
• Female
• Flavonoids (metabolism, pharmacology)
• Ischemia
• Malondialdehyde (metabolism)
• Mice
• Ovary (pathology)
• Oxidative Stress
• Rats
• Rats, Wistar
• Reperfusion Injury (drug therapy, metabolism, prevention & control)