Preliminary evaluation of selected inflammatory cytokine gene expression in lymphocytes isolated from whole human blood infected with trans-anethole-treated Staphylococcus aureus Newman strain.
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| Abstract |
In our previous study based on a whole-blood model of sepsis infected with trans-anethole (TA)-treated Staphylococcus aureus, we have found that innate immune response was more effective in comparison to non-treated cells. Due to the previous observation, in the current preliminary study, a primary adaptive immune response was analysed. This study was conducted to evaluate the expression of selected cytokine (IL1B, IL2, IL6, IL10, TNF, TGFB1, IFNG) and Toll-like receptor (TLR2) genes in lymphocytes isolated from whole human blood infected with S. aureus Newman strain treated with TA. The lymphocytes were isolated by density gradient centrifugation from blood samples infected with S. aureus, as well as from non-infected samples. Gene expression was measured using quantitative real-time PCR. The lymphocytes isolated from the blood infected with TA-treated staphylococcal cells demonstrated significantly greater IL10, IL1B, IL6, TNF and TLR2 expression. Hence, it is possible that the previously observed changes in the surface structure of TA-treated S. aureus Newman strain may significantly increase the relative expression of IL10, IL1B, IL6, TNF and TLR2 genes in lymphocytes; however, further studies are needed.
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| Authors | P Kwiatkowski, M Kurzawski, Ł Łopusiewicz, A Pruss, M Sienkiewicz, I Wojciechowska-Koszko, B Dołęgowska |
| Journal | Letters in applied microbiology (Lett Appl Microbiol) Vol. 74 Issue 4 Pg. 513-518 (Apr 2022) ISSN: 1472-765X [Electronic] England |
| PMID | 34904269 (Publication Type: Journal Article) |
| Copyright | © 2021 The Society for Applied Microbiology. |
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