Topical Administration of an Ointment Prepared From Satureja sahendica Essential Oil Accelerated Infected Full-Thickness Wound Healing by Modulating Inflammatory Response in a Mouse Model.

Abstract

INTRODUCTION:

Satureja sahendica has antibacterial and anti-inflammatory properties that can have beneficial effects for decreasing inflammation in infected wounds.

OBJECTIVE:

This study was conducted to evaluate the effects of an ointment prepared from S sahendica essential oil (SSO) on an infected wound model in BALB/c mice.

MATERIALS AND METHODS:

One full-thickness excisional skin wound was surgically created per animal and inoculated with 5 × 107 colony-forming units of Pseudomonas aeruginosa and Staphylococcus aureus. Following induction of the wound, the mice (N = 90) were treated with soft yellow paraffin (negative control, n = 18), mupirocin (positive control, n = 18) and 1%, 2%, and 4% SSO (n = 18 in each of the 3 groups). To determine the effect of the treatments on healing of an infected wound, the following factors were assessed: rate of the wound area, tissue bacterial count, histopathology, collagen biosynthesis, immunohistochemistry, and the expressions of insulin-like growth factor (IGF)-1, fibroblast growth factor (FGF)-2, vascular endothelial growth factor (VEGF), interleukin (IL)-1ß, IL-4, transforming growth factor beta (TGF-ß), and chemokine (CXC motif) ligand 1 (CXCL-1) on days 3, 7, and 14 after induction of the wound.

RESULTS:

Topical administration of SSO shortened the inflammatory phase, accelerated cellular proliferation, and increased fibroblast distribution per 1 mm2, collagen deposition, and rapid reepithelialization in comparison with control animals (P <.05). The messenger RNA levels of IGF-1, IL-10, FGF-2, VEGF, TGF-ß1, and CXCL-1 were remarkably increased, and IL-1ß level decreased (P <.05) in the treated animals compared with the control group (P <.05). The immunohistochemical analyses showed topical administration of SSO increased collagen biosynthesis in the treated group (P <.05).

CONCLUSIONS:

Topical administration of SSO shows evidence of accelerating wound healing by upregulating the expression of IGF-1, IL-10, FGF-2, VEGF, TGF-ß, and CXCL-1; shortening the inflammatory stage; and promoting the proliferative phase.

• Authors: Khaled Omarizadeh, Mohammad Reza Farahpour, Mahshid Alipour
• Journal: Wounds : a compendium of clinical research and practice (Wounds) Vol. 33 Issue 12 Pg. 321-328 (Dec 2021) ISSN: 1943-2704 [Electronic] United States
• PMID: 34882574 (Publication Type: Journal Article)

Chemical References

• Oils, Volatile
• Ointments
• Vascular Endothelial Growth Factor A

Topics

• Administration, Topical
• Animals
• Mice
• Mice, Inbred BALB C
• Oils, Volatile (pharmacology)
• Ointments
• Satureja
• Vascular Endothelial Growth Factor A
• Wound Healing