A Nano-Based P1Cminimum Probe to Monitor Thrombosis in Grafts and Improve Cardiac Function After Coronary Artery Bypass Grafting Surgery.

Abstract

Magnetic resonance imaging is widely used to identify and monitor thrombi in grafted vessels following coronary artery bypass grafting surgery (CABG). We produced a biosensor, P1Cm-SPIO-Cy5.5, composed of P1Cm peptide, superparamagnetic iron oxide nanoparticles (SPIO), and polyethylene glycol (PEG), for use in MRI thrombus imaging. Activated platelets induced by adenosine diphosphate were used for combination tests in vitro. A rat model of common carotid artery thrombosis was used for anti-thrombus experiments in vivo. P1Cm-SPIO-Cy5.5 remains stable in vitro and has good anticoagulation capacity. It can bind specifically to activated platelets and thrombi. In rats that underwent bypass surgery, P1Cm-SPIO-Cy5.5 could detect and label thrombi over a long period, and prevent thrombosis in grafted vessels. P1Cm-SPIO-Cy5.5 improved cardiac function in rats following CABG surgery. P1Cm-SPIO-Cy5.5 is a potential sensor for use in MRI for the early diagnosis and prevention of thrombosis after CABG surgery.

• Authors: Qiyong Wu, Xiaoqiang Tang, Haifeng Shi, Yong Zhang, Tao Wang, Ruohan Yin, Changjie Pan
• Journal: Journal of biomedical nanotechnology (J Biomed Nanotechnol) Vol. 17 Issue 10 Pg. 1951-1959 (Oct 01 2021) ISSN: 1550-7033 [Print] United States
• PMID: 34706795 (Publication Type: Journal Article)

Chemical References

• Peptides
• Polyethylene Glycols

Topics

• Animals
• Coronary Artery Bypass
• Magnetic Resonance Imaging
• Peptides
• Polyethylene Glycols
• Rats
• Thrombosis (diagnostic imaging, etiology)