Abstract | OBJECTIVE: METHODS: The study included 155 patients with COPD and 134 healthy volunteers. Genotyping was determined performing home-made polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The difference between the inhibitory activities of normal and oxidized Alpha-1-Antitrypsin (A1AT) against elastase and trypsin was used for determination of Oxidized Alpha-1-Antitrypsin ( OxyA1AT) (expressed as % and g/L). Functional activity of A1AT was presented as a specific inhibitor activity to elastase (SIA- Elastase, kU/g). RESULTS: Frequencies of the genotypes AA, AG and GG were 80.0%, 20.0%, 0% in COPD patients and 80.5%, 18.5% and 1.5% in the control group, and there was no significant difference in genotype or allele distributions between groups. Serum level of A1AT (g/L) and OxyA1AT was significantly higher in COPD patients than in the control group, but functional activity of A1AT (SIA- Elastase) was significantly lower in COPD patients than in the control group. In COPD group, increased level of OxyA1AT was present in G allele carriers who were smokers relative to G allele carriers who were not smokers. In the smoker group of patients with severe and very severe COPD (GOLD3+4), significant increase in OxyA1AT level was present in G allele carriers compared to AA homozygotes. CONCLUSION: These findings suggest that MSRA rs10903323 gene polymorphism is probably not a risk for COPD by itself but could represent a COPD modifier, since minor, G allele, is associated with an increased level of oxidized A1AT, indicating impaired ability of MSRA to repair oxidized A1AT in COPD-smokers, and in severe form of COPD.
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Authors | V Milovanovic, A Topic, N Milinkovic, Z Lazic, A Ivosevic, D Radojkovic, A Divac Rankov |
Journal | Pulmonology
(Pulmonology)
2024 Mar-Apr
Vol. 30
Issue 2
Pg. 122-129
ISSN: 2531-0437 [Electronic] Spain |
PMID | 34674978
(Publication Type: Journal Article)
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Copyright | Copyright © 2021 Sociedade Portuguesa de Pneumologia. Published by Elsevier España, S.L.U. All rights reserved. |
Chemical References |
- Methionine Sulfoxide Reductases
- Pancreatic Elastase
- methionine sulfoxide reductase
- SERPINA1 protein, human
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Topics |
- Adult
- Humans
- Methionine Sulfoxide Reductases
(genetics)
- Oxidative Stress
- Pancreatic Elastase
(genetics)
- Polymorphism, Genetic
- Pulmonary Disease, Chronic Obstructive
(genetics)
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