Carbon dots up-regulate heme oxygenase-1 expression towards acute lung injury therapy.

Abstract

Pneumonia is a kind of inflammation, which can cause high morbidity and mortality, and the treatment of pneumonia has received widespread attention. Heme oxygenase-1 (HMOX1) is a cell protective enzyme and can generate an anti-inflammatory response. Here, we demonstrate that degradable carbon dots (from L-ascorbic acid, CDs-1) can up-regulate the expression of HMOX1 in animal cells and tissues, which has a therapeutic effect on LPS-induced acute lung injury in mice. It was confirmed from in vitro experiments that CDs-1 could significantly up-regulate the expression of mRNA and the protein of HMOX1, which can increase the expression of HMOX1 by 5 times in a short time, decreasing the reactive oxygen species level in a cellular inflammation model induced by LPS. Furthermore, a series of in vivo comparative experiments show that CDs-1 could effectively treat acute lung injury and improve the survival rate of mice to 80%. Our work provides a practical way for the treatment of acute inflammation and the promising application of CDs in anti-inflammation.

• Authors: Bo Wang, Peipei Liu, Hui Huang, Xiting Wang, Mengling Zhang, Jian Huang, Fang Lu, Jian Chen, Yang Liu, Zhenhui Kang
• Journal: Journal of materials chemistry. B (J Mater Chem B) Vol. 9 Issue 43 Pg. 9005-9011 (11 10 2021) ISSN: 2050-7518 [Electronic] England
• PMID: 34617947 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lipopolysaccharides
• Membrane Proteins
• Reactive Oxygen Species
• Carbon
• Heme Oxygenase-1
• Hmox1 protein, mouse
• Ascorbic Acid

Topics

• Acute Lung Injury (chemically induced, drug therapy, metabolism)
• Animals
• Ascorbic Acid (chemistry, pharmacology)
• Carbon (chemistry, pharmacology)
• Female
• Heme Oxygenase-1 (genetics, metabolism)
• Lipopolysaccharides
• Membrane Proteins (genetics, metabolism)
• Mice
• Mice, Inbred BALB C
• Particle Size
• Quantum Dots (chemistry)
• RAW 264.7 Cells
• Reactive Oxygen Species (metabolism)
• Surface Properties
• Up-Regulation (drug effects)