Sinapic Acid Attenuates Rheumatoid Arthritis Through Reducing Inflammation and Oxidative Stress by Downregulating IκB Kinase.

Abstract

Sinapic acid (SA) was reported to protect against inflammation in various types of diseases. However, the role of SA in rheumatoid arthritis remains unclear. This study was designed to investigate the role of SA on rheumatoid arthritis. Rheumatoid arthritis mouse model was established by collagen immunization [collagen-induced arthritis (CIA)]. Histological analysis of articular cartilage tissue was carried out by hematoxylin and eosin (H&E) staining. Serum concentrations of tumor necrosis factor alpha and interleukin 6 were determined through enzyme-linked immunosorbent assay (ELISA). Oxidative damage indexes such as superoxide dismutase activity, malondialdehyde detection, glutathione detection, and catalase were determined by biochemical analysis. The protein levels of related genes were determined using Western blot. In CIA model, SA treatment attenuated paw swelling and clinical score of arthritis, attenuated articular cartilage tissues edema and infiltration of inflammatory cells, suppressed inflammatory cytokines release, and attenuated oxidative damage indexes. Mechanically, SA suppressed immune responses through inhibiting the IκB kinase (IKKs). SA attenuates rheumatoid arthritis through reducing inflammation and oxidative stress by downregulating IKKs.

• Authors: Long Wang, Fang Pan, Tao Luo
• Journal: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research (J Interferon Cytokine Res) Vol. 41 Issue 9 Pg. 347-354 (09 2021) ISSN: 1557-7465 [Electronic] United States
• PMID: 34543128 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Coumaric Acids
• Cytokines
• sinapinic acid
• Superoxide Dismutase
• I-kappa B Kinase

Topics

• Animals
• Antioxidants (metabolism)
• Arthritis, Experimental (chemically induced, drug therapy, metabolism)
• Arthritis, Rheumatoid (chemically induced, drug therapy, metabolism)
• Coumaric Acids (pharmacology)
• Cytokines (metabolism)
• Disease Models, Animal
• Down-Regulation (drug effects)
• I-kappa B Kinase (metabolism)
• Inflammation (chemically induced, drug therapy, metabolism)
• Male
• Mice
• Mice, Inbred DBA
• Oxidative Stress (drug effects)
• Superoxide Dismutase (metabolism)