A novel approach towards obesity: The use of a bacterial product, gassericin A, in 3T3-L1 cells.

Abstract

BACKGROUND AND OBJECTIVE:

The problem of obesity and its related complications are adversely affecting human society. We studied the effects of gassericin A, a bacteriocin produced by the intestinal bacteria, on adipocyte differentiation and development.

DESIGN:

Gassericin A was purified from Lactobacillus gasseri LA39 and was added to the culture medium of 3T3-L1 cells in two phases: Phase 1, 3T3-L1 cells were incubated with gassericin A while being induced to adipocytes (days 1-7); phase 2, the cells were incubated with the bacteriocin after being induced to adipocytes (days 8-12). The resultant changes in the pattern of expression of some of the important genes involved in adipogenesis were evaluated by RT-qPCR. The viability of cells and their numbers were also studied.

RESULTS:

In phase 1 of the study, the levels of transcripts for stearoyl CoA desaturase (SCD-1), zinc finger protein 423 (zfp-423), and glucose transporter 4 (GLUT4) genes were significantly reduced, while that of 422ap2 gene showed a significant increment (p < 0.05). In phase 2, the zfp-423 gene showed a reduction of expression and the 422ap2 gene showed an increase in expression (p < 0.05). The other genes including UCP-1 and TNF-α did not show any significant changes in neither of the groups. Gassericin A did not affect the morphology or viability of the cells, however, the numbers of cells had nearly doubled in the treatment groups.

CONCLUSION:

It seems that gassericin A could significantly alter the properties of adipocytes while they are in the process of development and after they have developed.

• Authors: Fereidoun Taghizad, Hamid Reza Kazerani, Hesam Dehghani, Ahmad Asoodeh, Danial Yaghubi
• Journal: Obesity research & clinical practice (Obes Res Clin Pract) 2021 Sep-Oct Vol. 15 Issue 5 Pg. 499-505 ISSN: 1871-403X [Print] Netherlands
• PMID: 34474970 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Asia Oceania Association for the Study of Obesity. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Bacteriocins
• gassericin A

Topics

• 3T3-L1 Cells
• Adipocytes (drug effects)
• Adipogenesis
• Animals
• Bacteriocins (pharmacology)
• Cell Differentiation
• Mice
• Obesity (therapy)