Genomic signatures of vegetable and oilseed allopolyploid Brassica juncea and genetic loci controlling the accumulation of glucosinolates.

Abstract

Allopolyploid Brassica juncea crops in Brassicaceae are becoming increasingly revitalized as vegetables and oilseeds owing to wide adaptability and significant economic values. However, the genomic differentiation of diversified vegetables and oilseed B. juncea and the genetic basis underlying glucosinolates accumulation have yet to be elucidated. To address this knowledge gap, we report the sequencing of pairwise genomes of vegetable and oilseed B. juncea at chromosome scale. Comparative genomics analysis unveils panoramic structural variation footprints, particularly the genetic loci of HSP20 and TGA1 associated with abiotic and biotic stresses responses between oilseed and vegetable subgroups. We anchored two major loci of MYB28 (HAG1) orthologues caused by copy number variations on A02 and A09 chromosomes using scored genomic SNPs-based GWAS that are responsible for seed oil quality-determining glucosinolates biosynthesis. These findings will provide valuable repertories of polyploidy genomic information enabling polyploidy genome evolution studies and precise genomic selections for crucial traits like functional components of glucosinolates in B. juncea crops and beyond.

• Authors: Jinghua Yang, Jing Wang, Zhangping Li, Xuming Li, Zhesi He, Lili Zhang, Tongyun Sha, Xiaolong Lyu, Sheng Chen, Yuanguo Gu, Zaiyun Li, Zhongyuan Hu, Hongju He, Ian Bancroft, Mingfang Zhang
• Journal: Plant biotechnology journal (Plant Biotechnol J) Vol. 19 Issue 12 Pg. 2619-2628 (12 2021) ISSN: 1467-7652 [Electronic] England
• PMID: 34448350 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

Chemical References

• Glucosinolates

Topics

• DNA Copy Number Variations
• Genetic Loci
• Genomics
• Glucosinolates
• Mustard Plant (genetics)
• Vegetables