Abstract |
Zinc biology, featuring intertwining signaling networks and critical importance to human health, witnesses exciting opportunities in the big data era of physiology. Here, we report a class of red- and far-red-emitting Zn2+ probes with Kd values ranging from 190 nM to 74 μM, which are particularly suitable for real-time monitoring the high concentration of Zn2+ co-released with insulin during vesicular secretory events. Compared to the prototypical rhodamine-based Zn2+ probes, the new class exploits morpholino auxochromes which eliminates phototoxicity during long-term live recording of isolated islets. A Si- rhodamine-based Zn2+ probe with high turn-on ratio (>100), whose synthesis was enabled by a new route featuring late-stage N-alkylation, allowed simultaneous recording of Ca2+ influx, mitochondrial signal, and insulin secretion in isolated mouse islets. The time-lapse multicolor fluorescence movies and their analysis, enabled by red-shifted Zn2+ and other orthogonal physiological probes, highlight the potential impact of biocompatible fluorophores on the fields of islet endocrinology and system biology.
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Authors | Junwei Zhang, Xiaohong Peng, Yunxiang Wu, Huixia Ren, Jingfu Sun, Shiyan Tong, Tianyan Liu, Yiwen Zhao, Shusen Wang, Chao Tang, Liangyi Chen, Zhixing Chen |
Journal | Angewandte Chemie (International ed. in English)
(Angew Chem Int Ed Engl)
Vol. 60
Issue 49
Pg. 25846-25855
(12 01 2021)
ISSN: 1521-3773 [Electronic] Germany |
PMID | 34423531
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2021 Wiley-VCH GmbH. |
Chemical References |
- Fluorescent Dyes
- Rhodamines
- Zinc
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Topics |
- Fluorescent Dyes
(chemistry, pharmacology)
- HeLa Cells
- Humans
- Insulin Secretion
(drug effects)
- Molecular Structure
- Rhodamines
(chemistry, pharmacology)
- Zinc
(chemistry, pharmacology)
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