Extraction and measurement of urinary tetranor-PGDM in disposable diapers.

Abstract

Urinary tetranor-PGDM is a useful diagnostic biomarker for food allergy which often affects infants. We attempted to extract and measure urinary tetranor-PGDM absorbed in polymer of diapers. We applied CaCl2 to the collected polymer, determined the adequate time length of shaking the polymer to release urine, and measured tetranor-PGDM in the extracted urine. This procedure provided high linearity and recovery rate in tetranor-PGDM measurement. We also found that urinary tetranor-PGDM was stable for 24 h at 4°C in diapers. This method can be useful to monitor the food allergic condition of non-toilet trained children.

• Authors: Nagisa Ito, Tatsuro Nakamura, Naoaki Sakamoto, Akane Hayashi, Takahisa Murata
• Journal: Journal of pharmacological sciences (J Pharmacol Sci) Vol. 147 Issue 2 Pg. 208-210 (Oct 2021) ISSN: 1347-8648 [Electronic] Japan
• PMID: 34384569 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 The Authors. Production and hosting by Elsevier B.V. All rights reserved.

Chemical References

• 11,15-dioxo-9-hydroxy-2,3,4,5-tetranorprostan-1,20-dioic acid
• Biomarkers
• Polymers
• Calcium Chloride
• Prostaglandin D2

Topics

• Biomarkers (urine)
• Calcium Chloride
• Child, Preschool
• Diapers, Infant
• Food Hypersensitivity (diagnosis)
• Humans
• Infant
• Liquid-Liquid Extraction (methods)
• Polymers
• Prostaglandin D2 (analogs & derivatives, isolation & purification, urine)
• Temperature
• Time Factors