Multidirectional anti-melanoma effect of galactolipids (MGDG-1 and DGDG-1) from Impatiens parviflora DC. and their synergy with doxorubicin.

Abstract

The anti-melanoma potential of galactolipids: MGDG-1 and DGDG-1, isolated from Impatiens parviflora, and their synergistic effect with anticancer drug - doxorubicin (DOX) was investigated. Both compounds demonstrated time- and dose-dependent cytotoxicity against human melanoma cells of different metastatic potential. MGDG-1 was more effective than DGDG-1, with the highest activity against A375 cell line (IC50 = 15.14 μg/mL). Both compounds acted selectively, were devoid of hepatotoxicity or mutagenicity. Additionally, MGDG-1 proved to be a tyrosinase inhibitor. Co-administration of MGDG-1 and DGDG-1 with DOX revealed a synergistic cytotoxic effect on melanoma cells. The cytotoxicity of all tested MGDG-1/DOX and DGDG-1/DOX cocktails was considerably higher than that of each agent administered alone. MGDG-1/DOX (Mix3) reduced the viability of A375 melanoma cells almost totally and this effect was 2-fold more potent as compared to DOX alone. Our study indicates that the overall effect is enhanced with the increasing concentration of MGDG-1 in the cocktail. These results open up a possibility for lowering therapeutic doses of chemotherapeutics such as doxorubicin when co-administrated with galactolipids. Thus, MGDG-1 can be prospectively considered as multidirectional anti-melanoma agent and can be recommended for further in vitro and in vivo studies, especially in search for effective combined therapy.

• Authors: Karolina Grabowska, Agnieszka Galanty, Paulina Koczurkiewicz-Adamczyk, Dagmara Wróbel-Biedrawa, Paweł Żmudzki, Daniel Załuski, Katarzyna Wójcik-Pszczoła, Paweł Paśko, Elżbieta Pękala, Irma Podolak
• Journal: Toxicology in vitro : an international journal published in association with BIBRA (Toxicol In Vitro) Vol. 76 Pg. 105231 (Oct 2021) ISSN: 1879-3177 [Electronic] England
• PMID: 34358613 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Chemical References

• 1-O-linolenoyl-2-O-hexadeca-7,10,13-trienoyl-3-O-galactopyranosylglycerol
• 3-O-(6-O-galactopyranosylgalactopyranosyl)-1,2-di-O-dodecanoylglycerol
• Antineoplastic Agents
• Biphenyl Compounds
• Glycolipids
• Picrates
• Doxorubicin
• 1,1-diphenyl-2-picrylhydrazyl
• Monophenol Monooxygenase

Topics

• Antineoplastic Agents (chemistry, pharmacology)
• Biphenyl Compounds (chemistry)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Doxorubicin (pharmacology)
• Drug Synergism
• Glycolipids (chemistry, pharmacology)
• Humans
• Impatiens
• Melanoma (drug therapy)
• Monophenol Monooxygenase (antagonists & inhibitors)
• Mutagenicity Tests
• Picrates (chemistry)
• Skin Neoplasms (drug therapy)
• Vibrio (drug effects, genetics)